Secretion of γ-Interferon at the Cellular Level |
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| Authors: | U ANDERSSON O MARTINEZ-MAZA J ANDERSSON S BRITTON H GAOLER M DE LEY S MODROW |
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| Institution: | Department of Immunobiology, Karolinska Institute, Wallenberg Laboratory, and Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden;Rega Institute, Division of Microbiology, Department of Human Biology, University of Leuven, Leuven, Belgium;and Max von Pettenkofer-Institute, Munich, FRG |
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| Abstract: | Using a haemolytic plaque assay for gamma-interferon (IFN-gamma) secretion we found that in vitro Epstein-Barr virus (EBV) exposure of peripheral blood mononuclear cells from EBV immune individuals led to IFN-gamma secretion, which was apparent within 6 h after virus contact and peaked 12-24 h after induction. Live, ultraviolet-light-irradiated and heat-inactivated virions all caused IFN-gamma secretion. In contrast, blood mononuclear cells from EBV non-immune adults or neonates could not be activated to IFN-gamma production by EBV. |
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