Supraoptic neurons in the rat hypothalamo-neurohypophysial explant: double-labeling with Lucifer Yellow injection and immunocytochemical identification of vasopressin- and neurophysin-containing neuroendocrine cells |
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Authors: | T A Reaves A Hou-Yu E A Zimmerman J N Hayward |
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Affiliation: | Department of Neuroanatomy, Institute of Brain Research, School of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113 Japan |
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Abstract: | By combining intracellular electrophysiology with double-labeled intracellular dye-marking and immunocytochemical identification of the same magnocellular neuroendocrine cell, we studied supraoptic neurons in the rat hypothalamo-neurohypophysial explant in vitro. This report examines neurophysiological and light microscopical features of vasopressin- and neurophysin-immunoreactive, pituitary-projecting supraoptic neurons. |
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Keywords: | wheat germ agglutinin-horseradish peroxidase conjugate thalamic posterior lateral nucleus cingulate gyrus cat |
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