爱普列特对原代培养大鼠输精管上皮细胞及bcl-2表达的影响

目的　探讨爱普列特对原代培养SD大鼠输精管上皮细胞及原癌基因bcl 2表达的影响。方法　应用HE染色、电镜、流式细胞术和免疫组化方法研究爱普列特对输精管上皮细胞形态改变、细胞凋亡率以及bcl 2表达的影响。结果　 0 1 μmol·L- 1 爱普列特对细胞形态无显著影响 ;0 3和 1 0 μmol·L- 1 爱普列特作用 72h后 ,细胞呈核固缩、深染、碎裂、染色质边集等多种形态改变 ,电镜下可见典型的凋亡细胞特征 ;流式细胞仪分析结果显示 ,溶剂对照组细胞凋亡率为 3 42 %± 1 49% ,0 1、0 3和 1 0 μmol·L- 1 爱普列特作用 72h后 ,细胞凋亡率分别为 4 66 %± 2 2 3 % ,39 0 4 %± 1 0 69%和 52 74%± 8 91 % ;免疫组化结果显示输精管上皮细胞bcl 2阳性率为 76 96 %± 9 2 5 % ,0 1、0 3和 1 0 μmol·L- 1 爱普列特作用 72h后 ,Bcl 2阳性表达的百分率分别为 63 93 %± 3 40 %、52 82 %± 8 66 %和 2 9 64 %± 8 74%。结论　 0 1 μmol·L- 1 爱普列特对大鼠输精管上皮细胞无影响 ,0 3和 1 0 μmol·L- 1 可诱导大鼠输精管上皮细胞发生凋亡 ,其作用机制可能与降低bcl 2表达有关

Effects of epristeride on vas deferens epithelial cells in rat and the expression of bcl-2

AIM To investigate the effects of epristeride on vas deferens epithelial cells in rat and the expression of bcl 2 METHODS Vas deferens epithelial cells were cultured in medium with variable doses of epristeride for 72 h The treated cells were examined under light microscope, transmission electron microscope and flow cytometry Immunohistochemistry was performed for detecting the expression of bcl 2 RESULTS There was no obvious morphological change on the cells after being pre treated with epristeride at 0 1 μmol·L -1 After treatment with epristeride at 0 3 and 1 0 μmol·L -1 , the cells became condensed and the chromatin clinged to the periphery of the nuclear Apoptotic bodies were observed The percentages of apoptotic cells were 3 42%±1 49%, 4 66%±2 23%, 39 04%±10 69% and 52 74%±8 91% respectively, after treatment with epristeride at 0, 0 1, 0 3 and 1 0 μmol·L -1 for 72 h The result of immunohistochemistry showed that there was a expression of bcl 2 on the cells. Epristeride may reduce the expression at 0 3 and 1 0 μmol·L -1 The expression percentages of bcl 2 on the cells were 76 96%±9 25%, 63 93%±3 40%, 52 82%±8 66% and 29 64%±8 74%, respectively, after being treated with epristeride at the concentration of 0, 0 1, 0 3 and 1 0 μmol·L -1 for 72 h CONCLUSION Pre treatment with epristeride at 0 1 μmol·L -1 results in no remarkable change in the cells Epristeride induces apoptosis on vas deferens epithelial cell in SD rat in vitro at the concentration of 0 3, 1 0 μmol·L -1 , and the mechanism may involve reducing the expression of bcl 2