中药喘可治对小鼠胸腺细胞抗凋亡作用研究

目的:研究中药喘可治(CKZ)对小鼠胸腺细胞的抗凋亡作用。方法:以地塞米松(DEX )诱导小鼠胸腺细胞凋亡建模,以CKZ保护小鼠胸腺细胞;6h时点以AnnexinV -FITC/PI双染流式细胞术检测早期凋亡和坏死细胞、以PI染色流式细胞术检测晚期凋亡。结果:CKZ +DEX组早期凋亡比率(3 5 2 9±2 73 ) %显著低于DEX组(4 5 97±1 3 9) %(P <0 0 1) ;该两组坏死细胞比率差异不显著。DEX +CKZ组晚期凋亡百分率为(17 2 6±4 5 8) % ,显著低于CKZ组(3 3 88±5 61) % (P <0 0 1)。上述各组数据与对照组差异显著(P <0 0 1)。结论:CKZ可以显著的抑制DEX诱导的小鼠胸腺细胞早期和晚期凋亡

Study on the Anti - apoptosis Effects of Chinese Herb Chuankezhi on Mouse Thymocyte

Objective :To study the anti apoptosis effect of Chinese herb Chuankezhi(CKZ) on mouse thymocyte. Methods :To establish dexamethasone(DEX) induced mouse thymocyte apoptosis model,to protect cells by CKZ;to detect early apoptotic and necrotic cells by Annexin V-FITC/PI double staining flowcytometry and to measure late apoptosis by PI staining method. Results :The early apoptotic rate of CKZ+DEX group(35 29±2 73)% was significantly lower the DEX group(45 97±1 39)%( P <0 01) and there was no significance for necrotic rates.The late apoptotic rate of DEX+CKZ group was (17 26±4 58)%,significantly lower than the CKZ group (33 88±5 61)%( P <0 01). Conclusion :CKZ can significantly inhibit DEX induced early and late apoptosis of mouse thymocyte apoptosis.