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| Bmi-1基因对人类胚胎纹状体来源神经干细胞凋亡的影响 | |
| 引用本文： | 赵春松,邹海强,闫晓明,陈凌,王佳茵,关云谦,张愚. Bmi-1基因对人类胚胎纹状体来源神经干细胞凋亡的影响[J]. 首都医科大学学报, 2015, 0(1): 103-110 |
| 作者姓名： | 赵春松 邹海强 闫晓明 陈凌 王佳茵 关云谦 张愚 |
| 作者单位： | 1. 首都医科大学宣武医院细胞生物室,北京100053; 教育部神经变性病重点实验室,北京100053 2. 广州军区广州总医院神经内科,广州,510010 3. 首都医科大学宣武医院功能神经外科,北京,100053 4. 首都医科大学宣武医院神经外科,北京,100053 |
| 基金项目： | 国家重点基础研究发展计划资助项目(973计划)(2012CBA01300);国家高技术研究发展计划资助项目(863计划)(2011AA020106);北京市自然科学基金(7102078);北京市科技新星项目(2009B22);北京市科委健康培育项目(Z111107067311033);北京市教育委员会科技计划重点项目(KZ201310025024)。This study was supported by National Program on Key Basic Research Project (2012CBA01300);National High Technology Research and Development Program of China (2011 AA020106);Natural Science Foundation of Beijing (7102078);Beijing Nova Program of Science and Technology (2009B22);Beijing Municipal Science and Technology Commission (Z111107067311033);Key Project of Beijing Municipal Education Com-mission Science and Technology Program(KZ201310025024)． |
| 摘 要： | 目的探讨Bmi-1基因对人类胚胎纹状体来源神经干细胞(neural stem cell,NSC)凋亡的影响。方法提取16~18周自然流产胚胎脑组织纹状体的NSC,体外培养至3~5代,用慢病毒载体对其进行Bmi-1基因的RNA干扰和过表达回复。观察Bmi-1基因对体外NSC凋亡的影响。结果在感染后2 h和3 d两个时间点,Bmi-1基因的RNA干扰显著增加了体外培养的NSC的凋亡率。结论 Bmi-1基因对于体外培养的NSC具有抑制其凋亡的作用,在分析该基因对NSC增生、自我更新等生物学属性的影响时,应当将Bmi-1基因的抗凋亡作用考虑在内。 |
| 关 键 词： | 神经干细胞 凋亡 Bmi-1 癌基因 |
Effect of Bmi-1 on apoptosis of human fetal striatum derived neural stem cells |
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| Zhao Chunsong,Zou Haiqiang,Yan Xiaoming,Chen Ling,Wang Jiayin,Guan Yunqian,Zhang YAlex. Effect of Bmi-1 on apoptosis of human fetal striatum derived neural stem cells[J]. Journal of Capital Medical University, 2015, 0(1): 103-110 | |
| Authors: | Zhao Chunsong Zou Haiqiang Yan Xiaoming Chen Ling Wang Jiayin Guan Yunqian Zhang YAlex |
| Affiliation: | Zhao Chunsong;Zou Haiqiang;Yan Xiaoming;Chen Ling;Wang Jiayin;Guan Yunqian;Zhang Y. Alex;Department of Cell Biology,Beijing Institute of Geriatrics,Xuanwu Hospital,Capital Medical University;Key Laboratory of Neurodegeneration,Ministry of Education;Department of Neurology,The General Hospital of Guangzhou Military Command in Guangzhou;Department of Function Neurosurgery, Xuanwu Hospital,Capital Medical University;Department of Neurosurgery,Xuanwu Hospital,Capital Medical University; |
| Abstract: | |
| Keywords: | neural stem cell apoptosis Bmi-1 oncogene |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |