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| HPLC同时测定掌叶半夏中四种含氮化合物的含量 | |
| 引用本文： | 徐立军,魏胜利,王文全,高雪岩,王俊英,李琳. HPLC同时测定掌叶半夏中四种含氮化合物的含量[J]. 中国中药杂志, 2011, 36(10): 1334-1336 |
| 作者姓名： | 徐立军 魏胜利 王文全 高雪岩 王俊英 李琳 |
| 作者单位： | 1. 北京中医药大学中药学院,北京,100102;石家庄市农林科学研究院,河北,石家庄,050041 2. 北京中医药大学中药学院,北京,100102;中药材规范化生产教育部工程研究中心,北京,100102 3. 北京中医药大学中药学院,北京,100102 4. 北京市农业技术推广站,北京,100102 |
| 摘 要： | 目的:用高效液相色谱法同时测定掌叶半夏中葫芦巴碱、胸腺嘧啶、腺嘌呤和6-氧嘌呤4种含氮化合物的含量.方法:采用Diamonil-C18色谱柱(4.6 mm×150 mm,5μm);乙腈-纯水-三乙胺梯度洗脱,流速1.0 mL·min-1;柱温25℃;检测波长254 nm.结果:葫芦巴碱在0.2～1.0μg(r:0.999 6),胸腺嘧啶在2.6～13μg(r=0.999 8),腺嘌呤在0.55～2.75μg(r=0.999 9),6-氧嘌呤在0.65～3.25μg(r:o.999 8)线性关系良好;葫芦巴碱平均同收率100.6%,RSD 2.4%(n=6);胸腺嘧啶平均回收率99.89%,RSD 0.20%(n=6);腺嘌呤平均回收率100.53%,RSD 1.1%(n=6);6-氧嘌呤平均回收率100.0%,RSD1.4%(n=6).结论:3批样品测定结果表明,该方法简便、准确,可用于掌叶半夏中4种含氮化合物的含鼍测定. |
| 关 键 词： | 掌叶半夏 含氮化合物 高效液相色谱 |
| 收稿时间： | 2010-08-30 |
Simultanenous determination of contents of four nitrogenous compounds in Pinellia peda-tisecta by HPLC |
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| XU Lijun,WEI Shengli,WANG Wenquan,GAO Xueyan,WANG Junying and LI Lin. Simultanenous determination of contents of four nitrogenous compounds in Pinellia peda-tisecta by HPLC[J]. China Journal of Chinese Materia Medica, 2011, 36(10): 1334-1336 | |
| Authors: | XU Lijun WEI Shengli WANG Wenquan GAO Xueyan WANG Junying LI Lin |
| Affiliation: | Chinese Medicine College, Beijing University of Chinese Medicine, Beijing 100102, China;Shijiazhuang Academy of Agriculture and Forestry Sciences, Shijiazhuang 050041, China;Chinese Medicine College, Beijing University of Chinese Medicine, Beijing 100102, China;Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs, Ministry of Education, Beijing 100102, China;Chinese Medicine College, Beijing University of Chinese Medicine, Beijing 100102, China;Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs, Ministry of Education, Beijing 100102, China;Chinese Medicine College, Beijing University of Chinese Medicine, Beijing 100102, China;Beijing Agricultural Technology Extension Station, Beijing 100102, China;Beijing Agricultural Technology Extension Station, Beijing 100102, China |
| Abstract: | |
| Keywords: | Pinellia peda-tisecta nitrogenous compounds HPLC |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |