海水浸泡对失血性休克大鼠心肌线粒体呼吸链的影响

目的 研究海水浸泡对失血性休克大鼠心肌细胞线粒体呼吸链的影响。方法 采用雄性Wistar大鼠24只，分为正常对照组、平原休克组和海水休克组，每组8只。测定血液动力学后用差速离心法分离心肌细胞线粒体，以氧电极技术和差光谱法测定琥珀酸呼吸链和还原型辅酶I(NADH)呼吸链的电子传递活性。结果 海水浸泡失血性休克大鼠血液动力学和心肌细胞线粒体琥珀酸—Co．Q还原酶、NADH细胞色素C还原酶、NADH—Co．Q还原酶、细胞色素氧化酶均显著低于对照组和平原休克组。结论 海水浸泡失血性休克大鼠心肌细胞线粒体呼吸链电子传递活性受到全面损伤，致使心肌氧利用发生障碍，可能是海水浸泡失血性休克大鼠心肌收缩功能下降、心输出量减少的原因之一。

Effects of Sea Water Immersing on Electron Transport Chains of Myocardial Mitochondria in Hemorrhagic Shock Rats

Objective To study the effects of sea water immersing on electron transportchains of myocardial mitochondria in hemorrhagic shock rats. Methods Twenty-four male Wistar rats were divided into 3 groups :control group(n = 8);hem-orrhagic shock group on land(groupA,n = 8) ;sea water immersing hemorrhagic shock group(groupB, n = 8). Contraction properties of cardiac muscle were investigated. Meanwhile,changes in electron transport chains of rat myocardial mitochondria were measured. Results The activities of succinate -Co. Q reductase,NADH -Co. Q reductase, NADH -cytochrome C reductase and cytochromi; oxidase in group B were significantly lower than those in control group and group A. Along with the electron transport chain alterations of myocardial mitochdria, there were decrease in myocardial contractile function. Conclusion The results showed that sea water immersing led to farther decrease in the activities of two respiratory chains. It also imply that weaknese of myocardial contractile function resulting in the decrease in cardiac output may be associated with the impairment of utilization of oxygen in myocardial mitochondria.