| HPLC法测定维吾尔药米黑尔给亚蜜膏中没食子酸的含量 |
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| 引用本文： | 热孜万古丽·阿不都热依木,杨晓琴. HPLC法测定维吾尔药米黑尔给亚蜜膏中没食子酸的含量[J]. 中国民族医药杂志, 2014, 0(9): 59-60 |
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| 作者姓名： | 热孜万古丽·阿不都热依木 杨晓琴 |
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| 作者单位： | 新疆维吾尔自治区喀什地区药品检验所,新疆喀什844000 |
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| 摘 要： | 目的：测定米黑尔给亚蜜膏中没食子酸的含量.方法：采用高效液相色谱法,Pheomenex luna C18色谱柱（250 ＆#215;4.60mm,5μm）柱温35℃,流动相为甲醇-0.2％磷酸（5：95）为流动相,检测波长为273nm,流速为1.0mL/min.结果：没食子酸在21.88～218.80μ g/mL间呈良好的线性关系,R2=0.9997,平均回收率为102.11％,RSD为0.53％.结论：该方法具有操作简单,灵敏,准确的优点,分离效果显著,可用于余甘子的含量测定和本产品质量控制.
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| 关 键 词： | 依本斯纳蜜膏 高效液相色谱法 没食子酸 含量测定 |
Determination of gallic acid content in miheier geya cream by HPLC |
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| Affiliation: | Reziwanguli· abduriyim YANG Xiao - qin ( Kashi drug control of xinjiang kashi 844000) |
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| Abstract: | Purpose： To determinate the contents of pyrogallic acid in miheiergeiyamigao. Method： An HPLC method was used and Pheomenex luna C18 was selected （250？ 4.60mm,5μm）, a column temperature was 35℃, methyl alcohol -0.2% Phosphoric acid （5：95 ） were mobile phase, the detecting wavelength and velocity were 273 nm and 1.0mL/min respectively. Results： There were established a good linear relation between 21.88 -218.80 μg/mL of pyrogallic acid, R2 =0.9997 the average recovery was 101.00%, RSD was 0.86 %. Conclusion： The methods had the advantages of simple and easy operation, sensitive and accurate and exhibited a good separation effect. The methods were can be used to determination of contents and quality control of the fructus phyllanthi. |
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| Keywords: | miheiergeiyamigao HPLC method Fructus phyllanthi Content determination |
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