水飞蓟宾磷脂复合物对高脂饮食大鼠脂肪肝和动脉硬化的影响

目的探讨水飞蓟宾磷脂复合物（水林佳）对高脂饮食大鼠脂肪肝和动脉硬化造模形成的影响。方法22只SD大鼠随机分为3组，对照组（n=6）普通饮食饲养，模型组（n=8）和干预组（n=8）以高脂饮食＋维生素D＋丙基硫氧嘧啶造模，干预组同时在饮水中加用水林佳（225mg·kg^-1·d^-1），各组大鼠均于实验第16周末处死，常规检测血液生化指标，并观察肝脏和主动脉壁病理改变。结果模型组出现明显的肝脏脂肪变、炎症和坏死，主动脉壁中膜轻度增厚，部分内皮下可见钙化斑，而水林佳干预组肝脏脂肪变和炎症程度较模型组明显改善（P〈0．05），且伴血清转氨酶水平显著下降（P〈0．01）。然而，水林佳干预组血糖、血脂及主动脉粥样硬化病变情况较模型组无明显改变。结论水林佳能减轻高脂饮食脂肪肝大鼠脂肪性肝炎程度，但无改善糖脂紊乱和动脉硬化作用。

The influence of silybin-phospholipid complex treatment on fatty liver disease and atherosclerosis in rats fed with high-fat diet

Objective To explore the effect of silybin-phospholipid complex treatment on fatty liver disease and atheroselerosis in rats fed with high-fat diet. Methods Twenty-two SD rats were randomly divided into three groups： the control group （n = 6）, the model group （n = 8 ） and the therapeutic group （n = 8 ）. The control group were fed with normal diet, and the other two groups were fed with high-fat diet plus Vitamin D and PTU. Meanwhile, the therapeutic group were treated with silybin Capsules （225 mg·kg^-1·d^-1）. All rats were sacrificed at the end of the 16th week of the experiment. The pathological changes in livers and aortas in all rats were pathologically evaluated with HE stain, and the serum biochemistry index was also measured. Results Significant hepatic steatosis, intra-lobule inflammation and spotty hepatocyte necrosis were seen in the model group. In addition, tunica media of aortas were slightly thickened and calcified plaque was observed under the endothelium in the model group. Compared with the model group, liver steatosis and inflammation were relieved in the therapeutic group pretreated with Silybin Capsules （P 〈 0.05） , and the serum levels of ALT were also significant decreased （P 〈 0.01 ）. However there was no difference on serum levels of fasting glucose and lipids, as well as lesions in aorta between the therapeutic group and the model group. Conclusion Silybin-phospholipid complex can improve the degree of steatohepatitis in rats induced by high-fat diet. However, it has no obvious benefit on hyperglycemia, dyslipidemia and aorta atherosclerosis