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Intraspecific variation in the structural organization and redundancy of chloroplast ribosomal DNA cistrons in Euglena gracilis
Authors:Edwin A Wurtz  Dennis E Buetow
Institution:(1) Department of Physiology and Biophysics, University of Illinois, 61801 Urbana, Illinois, USA;(2) Present address: Laboratory of Radiation Ecology, Fisheries Center, WH-10, University of Washington, 98105 Seattle, Washington, USA
Abstract:Summary Chloroplast DNAs from six different laboratory collections of Euglena gracilis ldquostrain Zrdquo and var. bacillaris were analyzed with restriction endonucleases EcoRI and Bam HI. The most variable portion of the organelle genome is the region containing the ribosomal cistrons. Intraspecific differences occur in both ribosomal DNA cistron number (one or three) and structural organization among those strains designated as ldquostrain Zrdquo and bacillaris. One culture previously designated as ldquoZrdquo is most likely bacillaris.
Keywords:Chloroplast  rDNA cistrons  Intraspecific variation  Euglena gracilis
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