强啡肽A对烫伤大鼠免疫功能与cAMP、cGMP含量变化的影响

采用放射免疫分析技术测定了强啡肽 A(DynA)对烫伤大鼠淋巴细胞内 cAMP 和 cGMP 含量的影响,并观察了其与免疫功能的关系,旨在探讨 DynA 调节烫伤大鼠免疫功能的机制。结果表明,烫伤大鼠脾淋巴细胞转化与白细胞介素2(IL-2)的生成明显降低,脾淋巴细胞内 cAMP 的含量明显升高,而 cGMP 的含量无明显变化。烫伤大鼠血清可抑制正常大鼠淋巴细胞转化功能和 IL-2的生成,并使淋巴细胞内 cAMP 含量升高。DynA 可增强烫伤大鼠淋巴细胞转化和 IL-2的生成,降低细胞内 cAMP 含量,钠洛酮可翻转 DynA 的这种作用。DynA 还可改善烫伤大鼠血清对淋巴细胞转化功能的抑制作用,降低烫伤大鼠血清引起的淋巴细胞内 cAMP 含量升高。提示 DynA 可增强烫伤大鼠的免疫功能,且这种作用与淋巴细胞内第二信使 cAMP 改变有关。

The effect of dynorphin A on intracellular cAMP and cGMP contents in spleen cells of burn rats and its relation to immune function

The lymphocyte response to Con A and IL-2 synthesis was clearly sup- pressed in rats after thermal injury.In burn rats,the cAMP content in lymphocytes was significantly increased,but cGMP level was not changed.The serum of burn rat could in- hibit Con A-induced lymphocyte proliferation and IL-2 production.The burn rat serum could also increase intracellular cAMP concentration.Dynorphin A enhanced lymphocyte proliferation and IL-2 synthesis,increased cAMP level in lymphocytes.Naloxone could inhibit the action of dynorphin A.Moreover,ynorphin A could partly reversed suppres- sion effect of burn serum on lymphocyte proliferation and suppressed the increase of in- tracellular cAMP content indueed by burn and rat serum.Our results suggested that dynorphin A augmented immune function of burn rats,and the action of dynorphin A was mediated by cAMP pathway.