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木香烃内酯对人乳腺癌SK-BR-3细胞增殖、迁移和凋亡的影响及机制研究
引用本文:马强,熊书,苗加伟,陈洁,周海英,罗娇,杨贞妮,孙厚良,邓雪松. 木香烃内酯对人乳腺癌SK-BR-3细胞增殖、迁移和凋亡的影响及机制研究[J]. 中国药房, 2020, 0(11): 1342-1347
作者姓名:马强  熊书  苗加伟  陈洁  周海英  罗娇  杨贞妮  孙厚良  邓雪松
作者单位:重庆三峡医药高等专科学校基础医学部;重庆市抗肿瘤天然药物工程技术研究中心;重庆三峡中心医院肾病学科
基金项目:重庆市自然科学基金资助项目(No.cstc2018jcyjAX0469);重庆市教育委员会科学技术研究项目(No.KJ1725385,No.KJQN201902701,No.KJQN201802711);重庆三峡医药高等专科学校校级项目(No.2016xzz03)。
摘    要:目的:研究木香烃内酯对乳腺癌SK-BR-3细胞增殖、迁移和凋亡的影响及机制。方法:取对数生长期的SK-BR-3细胞,分别加入不同浓度(10、20、30、40、50μmol/L)的木香烃内酯作用24、48、72 h,采用CCK-8法检测细胞的增殖抑制率。将细胞分为空白对照组和木香烃内酯10、20、30μmol/L浓度组,采用Hoechst 33258荧光染色法观察细胞形态及凋亡情况,并计算细胞凋亡率;采用细胞划痕试验检测细胞的迁移能力,并计算迁移率;采用Western blotting法检测细胞中B淋巴细胞瘤因子2(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱氨酸蛋白酶3(Caspase-3)和分裂型Caspase-3(Cleaved Caspase-3)蛋白的相对表达水平。结果:木香烃内酯对SK-BR-3细胞具有显著的增殖抑制作用(P<0.05或P<0.01),且呈现浓度和时间依赖趋势。空白对照组细胞轮廓清晰、形态规则、贴壁较好;与空白对照组比较,木香烃内酯10、20、30μmol/L浓度组细胞数量明显减少,细胞结构松散、体积缩小、间隙变大,且多数细胞轮廓消失、变圆,...

关 键 词:木香烃内酯  人乳腺癌SK-BR-3细胞  增殖  迁移  凋亡  机制

Study on the Effects of Costunolide on the Proliferation,Migration and Apoptosis of Human Breast Cancer SK-BR-3 Cells and Its Mechanism
MA Qiang,XIONG Shu,MIAO Jiawei,CHEN Jie,ZHOU Haiying,LUO Jiao,YANG Zhenni,SUN Houliang,DENG Xuesong. Study on the Effects of Costunolide on the Proliferation,Migration and Apoptosis of Human Breast Cancer SK-BR-3 Cells and Its Mechanism[J]. China Pharmacy, 2020, 0(11): 1342-1347
Authors:MA Qiang  XIONG Shu  MIAO Jiawei  CHEN Jie  ZHOU Haiying  LUO Jiao  YANG Zhenni  SUN Houliang  DENG Xuesong
Affiliation:(Dept.of Basic Medicine,Chongqing Three Gorges Medical College,Chongqing 404120,China;Chongqing Engineering Research Center of Antitumor Natural Drugs,Chongqing 404120,China;Dept.of Nephrology,Chongqing Three Gorges Center Hospital,Chongqing 404000,China)
Abstract:OBJECTIVE:To study the effects of costunolide on the proliferation,migration and apoptosis of breast cancer SK-BR-3 cells and its mechanism.METHODS:SK-BR-3 cells in logarithmic growth period were collected and cultured with different concentrations(10,20,30,40,50μmol/L)of costunolide for 24,48,72 h.Inhibitory rate of costunolide on cell proliferation was detected with CCK-8.The cells were divided into blank control group and costunolide group(10,20,30μmol/L).Hoechst 33258 fluorescence was used to observe the morphology and apoptosis of cells,and apoptotic rate of cells were calculated.Cell scratch test was used to detect the migration ability of cells and calculate the migration rate.Western blotting was used to detect the relative expression level of Bcl-2,Bax,Caspase-3 and Cleaved Caspase-3 in cells.RESULTS:The proliferation of SK-BR-3 cells were significantly inhibited by costunolide(P<0.05 or P<0.01),and it shows a trend of concentration and time dependence.In the blank control group,cells possessed clear contour,regular shape and good adherence.Compared with blank control group,the number of cells were decreased significantly in 10,20,30μmol/L costunolide groups,the cell structure was loose,the volume was reduced,and the gap became larger,and most of the cell contour disappeared and became round,the cell adherence was poor;cell migration rate and Bcl-2 protein relative expression level were decreased significantly,while apoptosis rate and the relative expression level of Bax,Caspase-3 and Cleaved Caspase-3 protein were significantly increased(P<0.05 or P<0.01).CONCLUSIONS:Costunolide can inhibit the proliferation and migration,and induce apoptosis of human breast cancer SK-BR-3 cells,mechanism of which may be through up-regulating the expression of Bax,Caspase-3 and Cleaved Caspase-3 while down-regulating the expression of Bcl-2.
Keywords:Costunolide  Human breast cancer SK-BR-3 cells  Proliferation  Migration  Apoptosis  Mechanism
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