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| 构建人乳头瘤病毒关键基因的原核重组载体 | |
| 引用本文： | 潘巍巍,赖国旗,宋方洲,易发平,马永平,左国伟.构建人乳头瘤病毒关键基因的原核重组载体[J].中国现代医学杂志,2006,16(21):3201-3204. |
| 作者姓名： | 潘巍巍 赖国旗 宋方洲 易发平 马永平 左国伟 |
| 作者单位： | 1. 重庆医科大学分子生物学教研室,重庆,400016 2. 重庆医科大学动物实验中心,重庆,400016 3. 重庆市生物化学与分子药理学重点实验室,重庆,400016 4. 教育部临床检验诊断学重点实验室,重庆,400016 |
| 基金项目： | 国家自然科学基金;重庆医科大学校科研和教改项目;重庆市教委资助项目;教育部春晖计划项目 |
| 摘 要： | 目的构建HPV16 E6／E7关键基因的原核载体pET-32（＋）-E6／E7并将其转到大肠杆菌BL-21（DE3）中诱导表达，最后获得E6／E7基因表达产物。方法用PCR方法从含有HPV16全基因序列的质粒中扩增E6／E7基因，将E6／E7基因连接到pET-32a（＋）质粒上，并转到大肠杆菌BL21（DE3）中。最后检测E6／E7基因。结果获得重组后的载体pET-32（＋）-E6／E7。结论成功构建表达了含有E6E7的原核重组载体，并在大肠杆菌中表达。 |
| 关 键 词： | 人乳头瘤病毒 HPV16E6/E7构建 重组载体 |
| 文章编号： | 1005-8982（2006）21-3201-04 |
| 收稿时间： | 2006-03-27 |
| 修稿时间： | 2006-03-27 |
Prokaryotic recombination vector construction of HPV important gene |
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| PAN Wei-wei,LAI Guo-qi,SONG Fang-zhou,YI Fa-ping,MA Yong-ping,ZUO Guo-wei.Prokaryotic recombination vector construction of HPV important gene[J].China Journal of Modern Medicine,2006,16(21):3201-3204. | |
| Authors: | PAN Wei-wei LAI Guo-qi SONG Fang-zhou YI Fa-ping MA Yong-ping ZUO Guo-wei |
| Institution: | 1.Department of Biochemistry and Molecular Biology, Chongqing University of Medical Sciences, Chongqing 400016, P.R.China; 2.Laboratory Centre, Chongqing University of Medical Sciences, Chongqing 400016, P.R.China; 3.Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing 400016, P.R.China; 4.Clinical Diagnostics Key Laboratory of the MOE, Department of Education, Chongqing 400016, P.R.China |
| Abstract: | Objective] To construct prokaryotic vector of HPV16 E6/E7 important gene, then transform it into BL-21(DE3) E.coli for expression and obtain the expressive production of HPVE6/E7. Methods] E6/E7 gene was amplified by polymerase chain reaction(PCR) from vector that contains all sequence of HPV16, the amplified fragnent E6/E7 gene was ligated into pET-32a (+) vector and transformed into BL-21(DE3)E.coli. E6/E7 gene was identified. Result] We obtained recombinant pET-32(+)-E6/E7 vector. Conclusions] We have successfully constructed vectors that have prokaryotic recombinant vector of E6/E7 gene which has been expressed in E.coli.. |
| Keywords: | human papilloma virus HPV16E6/E7 construction recombinant carrier |
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