Allelic structures at hypervariable minisatellite B6.7 in Japanese show population specificity |
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Authors: | Mizukoshi Tsunenori Tamaki Keiji Azumi Jun-ichi Matsumoto Hiroshi Imai Kohzoh Jeffreys Alec J |
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Institution: | (1) Department of Legal Medicine, Sapporo Medical University School of Medicine, South 1, West 17, Chuo-ku, Sapporo 060-8556, Japan Tel. +81-11-611-2111; Fax +81-11-611-3935 e-mail: tm13@pop01.odn.ne.jp, JP;(2) First Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan, JP;(3) Department of Genetics, University of Leicester, Leicester, UK, GB |
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Abstract: | Human minisatellite B6.7 shows extensive allele length and structural variability in north Europeans. We analysed this locus
in the Japanese population. Allele size distributions showed that Japanese retain extensive allele length variability but
have significantly smaller alleles compared with north Europeans. In contrast, there is very little variation in flanking
DNA, with only one single-nucleotide polymorphism (SNP) near the minisatellite. Ninety-two Japanese alleles were further characterised
by minisatellite variant repeat mapping by polymerase chain reaction (MVR-PCR). These alleles showed a wide variety of internal
MVR structures, despite their relative shortness, with most alleles observed only once in the sample. The true heterozygosity
is estimated at 99.95%, with well in excess of 2000 different alleles existing in the Japanese population. Dot matrix analysis
showed that groups of related alleles sharing structural motifs could be identified within Japanese and in north Europeans,
and that these groups are population specific with no examples of significant similarity between any Japanese and north European
alleles. Minisatellite B6.7 therefore shows huge allele variability and fast repeat turnover in Japanese as well as north
European populations, and provides novel lineage markers for exploring very recent events in human population history.
Received: January 29, 2002 / Accepted: February 12, 2002 |
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Keywords: | Minisatellite B6 7 MVR-PCR VNTR Mutation Human diversity |
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