中国阿昌族5个X-STR位点多态性研究

目的:调查X染色体上5个STR位点(DXS7130、DXS7132、DXS7133、DXS6804和DXS8378)在中国阿昌族群体的遗传多态性及其法医学方面的应用价值。方法:用PCR和变性聚丙烯酰胺凝胶电泳结合银染显带技术,检测100名阿昌族无关个体上述5个位点的等位基因频率及基因型分布,计算法医学常用参数,并对女性进行Hardy-Weinberg平衡检验。结果:5个位点在中国阿昌族群体中均具有遗传多态性,女性的基因型频率分布经χ2检验均符合Hardy-Weinberg平衡定律。结论:研究结果丰富了中华民族基因数据库,可以应用于法医学个体识别和亲权鉴定及群体遗传学研究。

Polymorphism of five X-STR loci in Chinese Achang ethnic group

Objective: To investigate the genetic polymorphism and applied value in forensic medicine of five short tandem repeats(STRs) loci(DXS7130,DXS7132,DXS7133,DXS6804 and DXS8378) on X-chromosome in Achang ethnic group. Methods: Allele frequency and genotype distribution of five X-STR loci among 100 unrelated individuals from Achang ethnic group were analyzed by PCR following polyacrylamide gel electrophoresis and silver staining.Hardy-Weinberg equilibrium of females was tested and forensic parameters were calculated. Results: All the five STR loci were polymorphic in Achang ethnic group.Chi-square test indicated that genotype distribution of females was in accordance with Hardy-Weinberg equilibrium. Conclusion: These results enrich Chinese genetic database and can be applied to individual identification,paternity testing and population genetics.