A new RT-PCR assay specific for influenza A(H2), multiplexed with an assay specific for HPAI A(H5N1) |
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| Authors: | C. Yea M. Petric J. Pasick R. Tellier |
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| Affiliation: | a Genetics and Genome Biology Program, Research Institute, Hospital for Sick Children, Canada;b British Columbia Centre for Diseases Control, Canada;c Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Canada;d Microbiology Division, Hospital for Sick Children, Canada;e Department of Laboratory Medicine and Pathobiology, University of Toronto, Canada |
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| Abstract: | Worldwide efforts are ongoing to improve influenza pandemic preparedness, including from the perspective of the clinical virology laboratory. In particular, much work has been devoted to the development of diagnostic assays targeted at the Highly Pathogenic Avian Influenza (HPAI) A(H5N1); much less efforts have been devoted to the A(H2) subtype. Yet, A(H2) subtype has a proven capacity to cause pandemics and is among the subtypes prioritized for surveillance and control. Although the human A(H2N2) virus that caused the pandemic of 1957 no longer circulates, many related avian A(H2) viruses circulate in avian population and could conceivably adapt to infect humans and cause a new pandemic.In this study the design and development of an RT-PCR assay specific for A(H2) subtype is presented. It is shown that the assay is highly sensitive and specific, able to detect human and avian A(H2) viruses, and can be incorporated into a multiplex assay with another previously described assay for HPAI A(H5N1). |
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| Keywords: | Influenza A virus Pandemic Influenza A(H2) RT-PCR |
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