心脑宁片对脑缺血大鼠乳酸、LDH、ATP酶活力的影响

目的 研究心脑宁片对大鼠脑缺血模型脑匀浆乳酸(lactic acid，LD)、乳酸脱氢酶(lactate dehydrogenase，LDH)水平及三磷酸腺苷(adenosine-triphosphate，ATP)酶活力的影响。方法 Wistar大鼠随机分为7组，每组12只：空白对照组，高、中、低剂量心脑宁片组、尼莫地平组、脑安片组和羧甲基纤维素钠组，测定脑匀浆中蛋白含量、LD、LDH、ATP酶水平。结果 大鼠脑缺血模型造模成功。与模型组相比，各剂量心脑宁片均可显著降低脑匀浆LD水平(P<0.01)，升高脑匀浆LDH活力(P<0.01)，并可显著升高脑匀浆Na⁺-K⁺-ATPase(P<0.01)、Mg²⁺-ATPase和Ca²⁺-ATPase活力(P<0.01或P<0.05)结论 心脑宁片具有明显改善脑缺血作用。

Effect of Xinnaoning Tablets on Lactic Acid, LDH and ATP in Cerebral Ischemia Rats

OBJECTIVE To study the effect of Xinnaoning tablets on lactic acid, LDH and ATPase activity effect in cerebral ischemia rats. METHODS Wistar rats were randomly divided into 7 groups: control group, model, high- middle- and low-dose of Xinnaoning tablets group, nimodipine group, Naoan tablets group and 0.5% CMC group. The content of brain homogenate protein, LD, LDH, ATPase levels were determined. RESULTS The model of cerebral ischemia rats were established successfully. Compared with model group, high-, middle-, and low- dose of Xinnaoning tablets could significantly decrease LD, raise LDH, and elevate brain homogenates of Na⁺-K⁺-ATPase, Mg²⁺-ATPase activity and Ca²⁺-ATPase activity (P<0.01 or P<0.05). CONCLUSION Xinnaoning tablets have significantly improve effect on cerebral ischemia.