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| 人乳牙牙髓干细胞体外表达和分泌胶质细胞源性神经营养因子的研究 | |
| 引用本文： | 王劲松,汪璇,王松灵.人乳牙牙髓干细胞体外表达和分泌胶质细胞源性神经营养因子的研究[J].北京口腔医学,2011,19(5):245-248. |
| 作者姓名： | 王劲松 汪璇 王松灵 |
| 作者单位： | 1. 100069,北京首都医科大学医学实验与测试中心 2. 首都医科大学基础医学院生理学系 教育部神经变性病重点实验室 3. 首都医科大学口腔医学院涎腺疾病中心及基因治疗牙再生分子生物学实验室 |
| 基金项目： | 国家自然科学基金(81000419、30500255); 北京市自然科学基金(5102010) |
| 摘 要： | 目的 研究人乳牙牙髓干细胞(SHED)表达和分泌胶质细胞源性神经营养因子(GDNF)的能力及规律,为进一步探讨SHED治疗帕金森病的作用机制提供依据.方法 通过酶消化法体外分离培养人乳牙牙髓中的干细胞,当传代至第3代时,加入神经干细胞的特殊培养基Neurobasal A和细胞因子进行神经球诱导培养,通过Real-Ti... |
| 关 键 词： | 人乳牙牙髓干细胞 神经球 胶质细胞源性神经营养因子 |
Expression and secretion of glial cell line-derived neurotrophic factors in stem cells from human exfoliated deciduous teeth in vitro |
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| WANG Jin-song,WANG Xuan,WANG Song-lin.Expression and secretion of glial cell line-derived neurotrophic factors in stem cells from human exfoliated deciduous teeth in vitro[J].Beijing Journal Of Stomatology,2011,19(5):245-248. | |
| Authors: | WANG Jin-song WANG Xuan WANG Song-lin |
| Institution: | WANG Jin-song,WANG Xuan,WANG Song-lin.Medical Experiment and Test Center,Capital Medical University,Beijing 100069,China |
| Abstract: | Objective To investigate the capability of stem cells from human exfoliated deciduous teeth(SHED) to produce and secrete glial cell line-derived neurotrophic factor(GDNF). Methods SHED were isolated from the pulp of normal exfoliated human deciduous incisors by enzymatic digestion and passage in vitro.At passage 3,SHED were induced by a medium optimized for neural stem cells growth which contained Neurobasal A and supplemented with other cell growth factors for neural differentiation.The expression of GDNF ... |
| Keywords: | Stem cells from human exfoliated deciduous teeth(SHED) Neurosphere Glial cell line-derived neurotrophic factor(GDNF) |
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