| RP-HPLC法测定男宝胶囊中补骨脂素和异补骨脂素含量 |
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| 引用本文： | 宋剑锋,张志根,张成川. RP-HPLC法测定男宝胶囊中补骨脂素和异补骨脂素含量[J]. 海峡药学, 2011, 0(8): 73-75 |
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| 作者姓名： | 宋剑锋 张志根 张成川 |
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| 作者单位： | 1. 浙江衢州市食品药品检验所 衢州324002
2. 浙江乐清市中医院 温州325000 |
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| 摘 要： | 目的建立一种可测定男宝胶囊中补骨脂素和异补骨脂素的RP-HPLC方法。方法采用Shim-pack VP-ODS（250mm×4.6mm 5μm）为色谱柱;流动相为甲醇-水（45∶55）;检测波长为246nm;流速为1.0mL.min-1;柱温为40℃;峰面积外标法定量;结果本法补骨脂素在9.10×10-4μg~0.2742μg范围内呈良好的线性关系,（r=0.999 99）,平均回收率为99.0%,RSD 1.57%;异补骨脂素在9.90×10-4μg~0.2970μg范围内呈良好的线性关系,（r=0.999 99）,平均回收率为100.7%,RSD 1.76%。结论本方法简便、快速、准确可靠,可作为该制剂的质控方法。
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| 关 键 词： | 男宝胶囊 补骨脂素 异补骨脂素 高效液相色谱法 含量测定 |
Determination of psoralen and isopsoralen in Nanbao capsules by RP-HPLC |
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| SONG Jian-feng,ZHANG Zhi-gen,ZHANG Cheng-chuan. Determination of psoralen and isopsoralen in Nanbao capsules by RP-HPLC[J]. Strait Pharmaceutical Journal, 2011, 0(8): 73-75 |
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| Authors: | SONG Jian-feng ZHANG Zhi-gen ZHANG Cheng-chuan |
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| Affiliation: | SONG Jian-feng1,ZHANG Zhi-gen1,ZHANG Cheng-chuan2(1.Quzhou Institute for Food and Drug Control,Quzhou 324002,Zhejiang,China,2.Yueqing Hospital of Traditional Chinese Medicine,Wenzhou 325200,China) |
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| Abstract: | OBJEICTIVE To establish a RP-HPLC method for determining the content of Psoralen and isopsoralen in Nanbao Capsules.METHODS A Shim-pack C18 column(250mm×4.6mm 5μm)was used.The mobile phase was methanol-water(45∶55).The detection wavelength was 246nm.RESULTS The linear range of paoralen was 9.10×10-4μg~0.2742μg(r=0.999 99).The average recovery(n=6) was 99.0%.RSD was 1.57%.The linear range of isopaoralen was 9.90×10-4μg~0.2970μg(r=0.99999).The average recovery(n=6) was 100.7%.RSD was 1.76%.CONCLUSION The method is simple,reliable,accurate and can be applied to the quality control of the preparetion |
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| Keywords: | Nanbao Capsules Psoralen Isopsoralen RP-HPLC Content determination |
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