| PRL-3基因RNA干扰慢病毒载体的构建及稳定感染HepG2单克隆细胞株的建立 |
| |
| 引用本文： | 黄德好,苏树英,陈规划.PRL-3基因RNA干扰慢病毒载体的构建及稳定感染HepG2单克隆细胞株的建立[J].中国临床药理学杂志,2011,27(11):862-866. |
| |
| 作者姓名： | 黄德好 苏树英 陈规划 |
| |
| 作者单位： | 1. 佛山市第一人民医院胆道外科,广东佛山,528000
2. 中山大学附属第三医院肝移植科,广州,510630 |
| |
| 基金项目： | 国家自然科学基金资助项目(30571769); 国家973重点基础研究发展规划基金资助项目(2009CB522404) |
| |
| 摘 要： | 目的 构建PRL -3基因RNA干扰慢病毒载体并建立被稳定感染的HepG2单克隆细胞株.方法 设计PRL -3基因特异性RNAi靶序列,合成靶序列的DNA oligo,退火形成双链DNA,与含绿色荧光蛋白(GFP)编码基因的pGCL- GFP线性化载体连接,产生重组慢病毒质粒LV - PRL3 - siRNA,转化感受...
|
| 关 键 词： | RNA干扰 慢病毒 PRL -3基因 肝细胞癌 |
Construction of a lentiviral vector for RNA interference of PRL-3 gene and establishment of monoclone of stably infected HepG2 cell lines |
| |
| HUANG De-hao,SU Shu-ying,CHEN Gui-hua.Construction of a lentiviral vector for RNA interference of PRL-3 gene and establishment of monoclone of stably infected HepG2 cell lines[J].The Chinese Journal of Clinical Pharmacology,2011,27(11):862-866. |
| |
| Authors: | HUANG De-hao SU Shu-ying CHEN Gui-hua |
| |
| Institution: | HUANG De-hao1,SU Shu-ying1,CHEN Gui-hua2 (1.Department of Biliary Surgery,The People's Hospital of Foshan,Foshan 528000,Guangdong Province,China,2.Department of Liver Transplantation,The Third Hospital Affiliated to Sun Yat-sen University,Guangzhou 510630,China) |
| |
| Abstract: | Objective To construct a lentiviral vector for PRL-3 gene RNA interference(RNAi) and establish monoclone of stably infected HepG2 cell lines.Methods The effective sequence of siRNA targeting PRL-3 gene was confirmed.Both sense and anti-sense DNA-oligo of the targeting sequence were designed,synthesized and cloned into the pGCL-GFP vector,which contained coding gene of green fluorescent protein(GFP).The resulting lentiviral vector containing PRL-3 siRNA was called LV-PRL3-siRNA,and it was confirmed by PCR an... |
| |
| Keywords: | RNA interference lentiviral PRL-3 gene hepatocellular carcinoma |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
