| UV—Vis法测定猕猴桃根提取物多糖的含量 |
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| 引用本文： | 何仁,杨苏芬,张英.UV—Vis法测定猕猴桃根提取物多糖的含量[J].海峡药学,2011,23(10):46-48. |
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| 作者姓名： | 何仁 杨苏芬 张英 |
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| 作者单位： | 浙江台州恩泽医疗中心(集团)台州医院 临海317000 |
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| 摘 要： | 目的建立从猕猴桃根中提取多糖，并测定多糖含量的方法。方法以UV-Vis法为检测手段．应用苯酚．硫酸法测定称猴桃根提取物多糖的含量。结果在10．02-60．12μg·mL-1范围内呈良好的线性关系，回归方程为：A=0．0159C＋0．028（r=0．9997），猕猴桃根多糖平均回收率为100．41％，RSD为1．10％．样品含量为56．74％，RSD为1．21％。结论本方法可靠、简便、准确，可用于称猴桃根中多糖的含量测定。
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| 关 键 词： | 猕猴桃根 多糖 苯酚-硫酸法 含量测定 |
Determination of polysaccharide in Kiwi fruit root by UV-Vis |
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| HE Ren,YANG Su-fen,ZHANG Ying.Determination of polysaccharide in Kiwi fruit root by UV-Vis[J].Strait Pharmaceutical Journal,2011,23(10):46-48. |
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| Authors: | HE Ren YANG Su-fen ZHANG Ying |
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| Institution: | HE Ren,YANG Su-fen,ZHANG Ying(Taizhou ENZE medical center(group) Taizhou Hospital,Linhai 317000,China) |
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| Abstract: | OBJECTIVE To establish a method for extracting the polysaccharide from Kiwi fruit root and determinate the content of polysaccharide.METHODS Determination the polysaccharide in kiwifruit root extracting by UV-vis and phenol-sulfuric.RESULTS In 10.02~60.12μg·mL-1 range is good linear relationship,the regression equation is:A=0.0159C+0.028(r=0.9997),The average recovery of Kiwi fruit root polysaccharide is 100.41%,RSD is 1.10%,and the sample content is 56.74%,RSD is 1.21%.CONCLUSION The method is reliable,simple,accurate and can be applied to determine the polysaccharide in Kiwi fruit root. |
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| Keywords: | Kiwi fruit root Polysaccharide phenol-sulfuric method Content determination |
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