高效液相色谱法测定入血浆中氟西汀浓度

目的建立测定人血浆中氟西汀浓度的高效液相色谱法。方法以DiamonsilTMC18反相柱（150mm×4．6mm，5μm）为色谱柱，流动相为0．03mol·L-1醋酸铵．甲醇（27：73）；流速：0．8mL·min-1；柱温：40℃；检测波长：260nm。以乙酸乙酯与二氯甲烷（80：20）为提取剂。结果氟西汀的低、中、高（10．0。40．0。320．0ng·mL-1）3种浓度平均回收率分别为101．56％，98．61％。97．92％。提取回收率分别为71．12％，73．71％，76．59％；日内、日间差RSD均低于9％（n=5）；分析方法的检测限为5．0ng·mL-1；线性范围为10．0--320．0ng·mL-1。线性方程：Y=98．12X＋1．71．r=0．9989（n=8）。结论该方法灵敏、准确、简单、快速，可用于氟西汀临床血药浓度监测和药动学研究。

Determination of the concentration of Fluoxetine in human plasma by HPLC

OBJECTIVE To establish a method for determining the concentration of fluoxetine in human plasma by HPLC.METHODS The drug from plasma was analyzed in a reverse phase HPLC system C18 column(150mm×4.6mm,5μm);mobile phase consisted of 0.03mol·L-1 ammonioum-methanol(27∶73);the flow rate was 0.8mL·min-1;the detection wavelength was at 260nm.Ethyl acetate and dichloromethane(80∶20)was used as extracting solvent.RESULTS The average recoveries of fluoxetine in low,middle and high concentrations(10.0,40.0,320.0ng·mL-1) were 101.56%,98.61%and 97.92%,respectively.The intra-day and inter-day variation(RSD)was less than 9%(n=5).The calibration curve of fluoxetine showed good linearity,r=0.9989(n=8),over the range of 10.0~320.0ng·mL-1.The regression equation was Y=98.12X+1.71,r=0.9989(n=8).The minimum detectable concentration of fluoxetine was 5.0ng·mL-1.CONCLUSION The method is sensitive,accurate,simple and quick,which can be used for clinical drug monitoring and pharmacokinetics studies of fluoxetine.