| HPLC法测定半夏中鸟苷的含量 |
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| 引用本文： | 卢晓萍,廖凤霞,胡元发.HPLC法测定半夏中鸟苷的含量[J].海峡药学,2011,0(8):78-79. |
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| 作者姓名： | 卢晓萍 廖凤霞 胡元发 |
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| 作者单位： | 1. 重庆桐君阁股份有限公司 重庆 400012
2. 重庆太极集团有限公司 重庆401147 |
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| 摘 要： | 目的建立半夏中鸟苷的含量测定方法。方法采用高效液相色谱法,色谱柱为Agilent Zorbax SB-C18（4.6×250mm,5μm）,流动相为甲醇：0.01%醋酸（3∶97）;检测波长254nm;流速为1.0mL.min-1。结果本法精密度高,稳定性好,鸟苷溶液在0.00411~0.0411mg.mL-1的浓度范围内具有良好的线性关系（r=0.9998）,平均加样回收率为103.26%,RSD%为2.46（n=6）。结论本方法简便,准确,重现性好,可作为半夏的含量测定方法。
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| 关 键 词： | 半夏 鸟苷 高效液相色谱法 |
Determination of Guanosine in Pinellia ternate Breit by HPLC |
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| LU Xiao-ping,LIAO Feng-xia,HU Yuan-fa.Determination of Guanosine in Pinellia ternate Breit by HPLC[J].Strait Pharmaceutical Journal,2011,0(8):78-79. |
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| Authors: | LU Xiao-ping LIAO Feng-xia HU Yuan-fa |
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| Institution: | LU Xiao-ping1,LIAO Feng-xia2,HU Yuan-fa2(1.Chongqing Tongjunge limited group,Chongqing 400012,China,2.Chongqing Taiji limited group,Chongqing 401147,China) |
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| Abstract: | OBJECTIVE To establish a method for determination of in Pinellia ternate Breit.METHODS Using a HPLC method with Agilent Zorbax SB-C18(4.6×250mm,5μm) column;the mobile phase was methanol 0.01% ethanoic acid(3∶97);the detection wavelength was set at 254nm;flowrate was 1.0mL·min-1;The precision and stability were fine1the linean range within 0.00411~0.0411mg·mL-1,r=0.9998.The average rocoveries of dihydromyricetine(n=6) was 103.26% with RSD 2.46%.CONCLUSION The method was simple,accurate and reproducible,and can be used to determine theGuanosine in Pinellia ternate Breit. |
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| Keywords: | Pinellia ternate Breit Guanosine HPLC |
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