超高效液相色谱法测定三七中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的含量

目的建立超高效液相色谱（UPLC）法测定三七中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的含量。方法采用ACQUITY BEH C18（2.1 mm×50 mm,1.7μm）色谱柱;流动相为乙腈-水,梯度洗脱;流速为0.4 mL·min^-1;检测波长为203 nm;柱温为35℃;进样体积为2μL。结果三七中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1分别在0.116 8～1.168μg、0.123 6～1.236μg、0.030 0～0.300μg范围内与相应峰面积呈良好线性关系,平均加样回收率分别为100.1%、99.3%、99.9%,RSD值分别为0.4%、0.6%、1.0%（n=6）。结论较之HPLC法,UPLC法在不影响分离效果的情况下可大大提高三七中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的分析速度,改善分析效果;同时可减少溶剂的消耗。UPLC法可作为替代传统HPLC法的一种更为方便、快捷、可靠的方法。

UPLC determination of ginsenoside Rg_1,ginsenoside Rb_1 and notoginsenoside R_1 in Panax Notoginseng

Objective To establish the quantitative method of ginsenoside Rg1,ginsenoside Rb1 and notoginsenoside R1 in Panax Notoginseng by ultra performance liquid chromatography（UPLC）.Methods This assay was performed on ACQUITY BEH C18（2.1 mm × 50 mm,1.7 μm） column with acetonitrile-water as mobile phase in gradient elution at a flow rate of 0.4 mL·min^-1And the detection wavelength was 203 nm.The column temperature was 35 ℃.The injection volume was 2 μL.Results Ginsenoside Rg1,Rb1 and notoginsenoside R1 in Panax Notoginseng had good linearity within the range of 0.116 8-1.168,0.123 6-1.236,0.030 0-0.300 μg,respectively.And their mean recovery rations were 100.1%,99.3%,99.9%with RSD of 0.4%,0.6%,1.0%（n=6）.Conclusion UPLC method may greatly improve the separation efficiency and analysis speed in the case of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Panax Notoginseng while reducing the solvent consumption.As an alternative of conventional HPLC,UPLC is more convenient,rapid and feasible.