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| 人参皂甙Rh_2抗乳腺癌细胞细胞侵袭和转移的实验研究 | |
| 引用本文： | 朴丽花,金政,蔡英兰,张默函,许祖德,韩春姬.人参皂甙Rh_2抗乳腺癌细胞细胞侵袭和转移的实验研究[J].中国临床药理学杂志,2011,27(11). |
| 作者姓名： | 朴丽花 金政 蔡英兰 张默函 许祖德 韩春姬 |
| 作者单位： | 1. 延边大学组织胚胎学教研室,吉林延吉,133000 2. 复旦大学附属华山医院病理科,上海,200040 3. 延边大学医学院预防医学教研部,吉林延吉,133000 |
| 摘 要： | 目的观察人参皂甙Rh2对人乳腺癌MCF7/Adr细胞侵袭和迁移的作用及其机制。方法用MTT比色法,检测人参皂甙Rh2(Rh2)对MCF7/Adr细胞的活力;Transwell小室测定其侵袭力和迁移力;Western blot法检测细胞基质金属蛋白酶2(MMP2)、MMP9和核转录因子KappaB(NF-KB)蛋白水平的变化。结果用人参皂甙Rh2处理后,MCF7/Adr细胞生长受到抑制,且作用呈时效-量效依赖关系;同时细胞侵袭和迁移能力明显降低;MMP2、MMP9和NF-kB蛋白的表达均明显减弱。结论人参皂甙Rh2能够减弱MCF7/Adr细胞侵袭和转移,其机制可能与降低MMP2、MMP9和NF-kB蛋白表达有关。 |
| 关 键 词： | 乳腺癌 人参皂甙Rh2 侵袭 转移 |
Experimental study on Gensenoside Rh_2 inhibiting the invasion and migration of breast cancer cells |
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| PIAO Li-hua,JIN Zheng,CAI Ying-lan,ZHANG Mo-han,XU Zu-de,HAN Chun-ji.Experimental study on Gensenoside Rh_2 inhibiting the invasion and migration of breast cancer cells[J].The Chinese Journal of Clinical Pharmacology,2011,27(11). | |
| Authors: | PIAO Li-hua JIN Zheng CAI Ying-lan ZHANG Mo-han XU Zu-de HAN Chun-ji |
| Institution: | PIAO Li-hua1,JIN Zheng1,CAI Ying-lan1,ZHANG Mo-han1,XU Zu-de2,HAN Chun-ji3 (1.Department of Anatomy and Histology and Embryology,Medical College of Yanbian University,Yanji 133000,Jilin Province,China,2.Department of Pathology,Huashan Hospital,Fudan University,Shanghai 200040,3.Department of Preventive Medicine,College of Basic Medicine,Yanbian University,China) |
| Abstract: | Objective To explore the effect of Gensenoside Rh2 and its mechanism on the invasion and migration of human breast cancer MCF7ArR cells.Methods The effect of Gensenoside Rh2 on cell viability was tested by MTT.The cell invasion and migration were determined by transwell chamber modcl.The expression of matrix metalloproteinases 2(MMP-2),MMP9,and nuclear factor-kappa B(NF-kB) were detected by Western blot.Results After being treated with Gensenoside Rh2,the proliferation of MCF7/AdrR cells was inhibited in a ... |
| Keywords: | breast cancer Gensenoside Rh2 invasion metastasis |
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