纳豆多糖改善小鼠IgA肾病的作用及其免疫学机制的研究分析

目的 探讨纳豆多糖改善小鼠IgA肾病的作用及其免疫学机制.方法 将造模成功的50只IgA肾病小鼠分为模型组,纳豆多糖高、中、低剂量组及阳性组(雷公藤多苷),每组10只.同时选择10只正常小鼠(未经造模)作为对照组.正常组和模型组小鼠按0.2 mL/10g 灌胃生理盐水(NS),阳性组(雷公藤多苷,5mg/kg),纳豆多糖高、中、低剂量组(给药剂量分别为40 、20、10 g/kg),1次/d,至第20周处死,取血及脏器,测定白蛋白、尿素氮、肌酐、血清循环免疫复合物和IgA水平.结果 与正常组比较,模型组尿微量白蛋白明显升高(P<0.05);与模型组比较,纳豆多糖高、中剂量组和阳性组尿微量白蛋白明显降低(P<0.05);与正常组比较,模型组尿血清白蛋白明显降低(P<0.05),与模型组比较,纳豆多糖高、中剂量组和阳性组血清白蛋白明显升高(P<0.05);与正常组比较,模型组尿素氮、肌酐、血清循环免疫复合物明显升高(P<0.05),与模型组比较,纳豆多糖高、中剂量组和阳性组尿素氮、肌酐、血清循环免疫复合物明显降低(P<0.05);与正常组比较,模型组肾组织IgA水平明显升高(P<0.05),与模型组比较,纳豆多糖高、中剂量组和阳性组IgA水平明显降低(P<0.05).结论 纳豆多糖可明显改善小鼠IgA肾病,还可降低尿蛋白和IgA水平.

Study of Natto Polysaccharide Effect and Immunological Mechanismof IgA Nephropathy in Mice

Objective To observe the protective effect of natto polysaccharide on IgA nephropathy mice and its immunological mechanism.Methods The IgA model mice were divided into model group, high-, medium-and low-doses of natto polysaccharide groups, and positive group.Each group had 10 mice.10 normal mice were enrolled as normal control group.The mice in normal control and model groups were treated with normal saline, and all doses of natto polysaccharide groups and positive group were treated with natto polysaccharide (40, 20, 10 g/kg) and positive (5mg/kg) respectively.All mice were treated with drugs or NS for 20 weeks, ig, qd.The levels of albumin in serum and urine, urea nitrogen, creatinine, CIC and IgA were investigated in each group.Results Compared with the normal control group, the levels of albumin in urine in model group were obviously higher (P<0.05).but compared with the model group, the levels of albumin in urine in high-, medium-doses of natto polysaccharide groups and positive group were obviously lower (P<0.05).Compared with the normal control group, the levels of albumin in serum in model group were obviously lower (P<0.05), but compared with the model group, the levels of albumin in serum in high-, medium-doses of natto polysaccharide groups and positive group were obviously higher (P<0.05).Compared with the normal control group, the levels of urea nitrogen, creatinine and CIC in model group were obviously higher (P<0.05), but compared with the model group, the levels of urea nitrogen, creatinine and CIC in high-, medium-doses of natto polysaccharide groups and positive group were obviously lower (P<0.05).Compared with the normal control group, the levels of IgA in model group were obviously higher (P<0.05), but compared with the model group, the levels of IgA in high-, medium-doses of natto polysaccharide groups and positive group were obviously lower (P<0.05).Conclusion Natto polysaccharide has protective effect on IgA nephropathy mice, and can reduce the levels of albumin and IgA.