| 限制性内切酶EcoRI的分离纯化 |
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| 引用本文： | 池木根. 限制性内切酶EcoRI的分离纯化[J]. 军事医学科学院院刊, 1995, 0(2) |
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| 作者姓名： | 池木根 |
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| 作者单位： | 军事医学科学院毒物药物研究所 |
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| 摘 要： | 以大肠杆菌ＲＹ＿（１３），Ｋ＿（１２１１００）为酶源菌株，采用聚乙烯亚胺分离纯化了限制性内切酶ＥｃｏＲＩ，并对λ－ＤＮＡ有５个专一的切割位点，将λ－ＤＮＡ切割成６个分子量不同的片段，经琼脂糖凝胶电泳后，可得６个迁移位置不同的条带。这些特性使它特别适合于作为ＤＮＡ结构功能等方面研究的工具酶。在遗传工程研究中，限制酶更起了决定性作用。
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| 关 键 词： | 限制性内切酶；EcoRI；聚乙烯亚胺；纯化 |
Isolation and purification of EcoR I restriction endonuclease |
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| Chi Mugen. Isolation and purification of EcoR I restriction endonuclease[J]. Bulletin of the Academy of Military Medical Sciences, 1995, 0(2) |
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| Authors: | Chi Mugen |
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| Abstract: | EcoR I restriction endonuclease was isolated from Escherichia coli RY_(13) andK_(121100).The puriflcation process involved treatment with ammonium sulfate, polyethyleneimineand phosphocellulose chromatography.By this method a highly purified EcoR I restrictionendonuclease can be prepared.The enzyme recognizes unique sites on DNA,with the minimalrecognition site being a hexanucleotide sequence characterized by 2-fold symmetry.Thus,theEcoR I enzyme is one of the simplest sequence-speciflc DNA enzyme known and is well suited toa study of DNA sequence recognition by proteins. |
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| Keywords: | restriction endonuclease EcoR I polyethyleneimine purification |
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