广东省人免疫缺陷病毒1亚型基因序列特征分析

目的研究广东省人免疫缺陷病毒1（HIV-1）亚型的基因序列特征。方法2002年采集100份HIV-1感染者的外周静脉抗凝血,分离单核细胞（PBMC）,提取前病毒DNA,通过聚合酶链反应（PCR）扩增,获得包膜蛋白（ENV）区基因的核酸片段,并对其C2～V3及邻区核苷酸序列进行测定和分析。所得序列用GCG软件包进行系统进化树和氨基酸变异分析。结果经过离散率计算和系统进化树分析后证实,其PCR扩增阳性的75份样品中,44份（58．7％）为重组毒株CRF01-AE（circulating recombinant form 01-AE）,27份（36％）为CRF—BC,4份为泰国B’亚型（5．3％）。结论广东省HIV-1以CRF01-AE和CRF—BC为主,也存在泰国B’亚型。目前我省境内有多种亚型存在,流行趋势日益严峻。加强艾滋病患者的治疗,以及控制和预防艾滋病的传播是今后面临的重要任务。

Subtype and sequence analysis of human immunodeficiency virus type-1 strains in Guangdong province

Objective To investigate subtype and genetic analysis of human immunodeficiency virus type-1 (HIV-1).Methods DNA sequences were amplified by nested-PCR from uncultured peripheral blood mononuclear cells(PBMC) obtained from 100 HIV-1 patients from Guangdong Province.The C2 to V3 region of the envelope glycoprotein gp120 of HIV-1 was sequenced directly.The analysis of the gene-based phylogenetic tree and variation of amino acid were carried out by using Wisconsin software package or genetics computer group(GCG).Results DNA fragments were amplified from 75 PBMC samples by using nested polymerase chain reaction (PCR).Sequence analysis showed that there were 3 HIV-1 subtypes or circulating recombinant forms(CRF) :CRF01-AE(n=44),CRF-BC(n=27) and B'(n=4).Condusion Three HIV-1 subtypes or circulating recombinant forms:CRF01-AE ,CRF-BC and B'might be circulating in Guangdong Province.Findings from this study suggested that several subtypes might exist in Guangdong Province and the epidemic situation of AIDS be serious.It should be a challenge for Gnangdong Province in treating patients,preventing and controlling AIDS in the future.