荆芥穗多糖的免疫调节活性

目的研究荆芥穗多糖(HSP)对Raw264.7细胞和免疫抑制小鼠的免疫调节活性。方法通过水提醇沉法提取HSP,苯酚-硫酸法测定HSP的总糖含量,硫酸-四硼酸钠法测定糖醛酸含量,考马斯亮蓝染色法测定蛋白质含量,高效液相色谱(HPLC)分析单糖组成;体外分别采用噻唑蓝(MTT)法和流式细胞术检测HSP对Raw264.7细胞增殖及吞噬能力的影响;体内通过环磷酰胺诱导建立免疫抑制小鼠模型,考察其对免疫抑制小鼠体质量增加值、胸腺指数、脾脏指数以及派氏结个数的影响。结果HSP含D-甘露糖1.73%、L-鼠李糖4.36%、D-葡萄糖醛酸1.38%、L-半乳糖醛酸6.67%、D-葡萄糖0.40%、D-半乳糖2.03%、L-阿拉伯糖92.26%,200,400,800μg·mL^-1HSP显著促进巨噬细胞增殖(P<0.05或P<0.01),显著增强巨噬细胞的吞噬活性(P<0.01),显著影响Raw264.7细胞形态,HSP能够显著提高免疫抑制小鼠的脾脏指数和胸腺指数(P<0.05)。结论HSP对免疫系统具有一定的调节作用。

Immunomodulatory Activity of Polysaccharides from Herba Schizonepetae

Objective To study the immunoregulatory activity of Herba schizonepetae polysaccharide(HSP)on Raw264.7 cells and immunosuppressive mice.Methods HSP was extracted by hot water and then precipitated in alcohol.Total sugar content was determined by phenol-sulfuric acid method.Uronic acid content was determined by sulfuric acid-sodium tetraborate method.Protein content was determined by Coomassie bright blue staining method,and monosaccharide composition was analyzed by HPLC.The effects of HSP on the proliferation and phagocytosis of Raw264.7 cells were detected by MTT and flow cytometry,respectively.The immunosuppressive mice model were induced by cyclophosphamide to investigate the effects of HSP on weight gain,thymus index,spleen index and PPs.Results There were 1.73%of D-mannose(Man),4.36%of L-rhamnose(Rha),1.38%of D-glucuronic acid(GlcA),6.67%of L-galacturonic acid(GalA),0.40%of D-glucose(Glc),2.03%of D-galactose(Gal),and 92.26%of L-arabinose(Ara)in HSP.HSP at the concentrations of 200,400,and 800μg·mL^-1 could promote the proliferation and phagocytosis of Raw264.7 cells(P<0.05 or P<0.01).It also could significantly affect the morphology of macrophages,and regulate the spleen and thymus index of immunosuppressed mice(P<0.05).Conclusion HSP has immunomodulatory effects on immune system.