肿瘤转移抑制基因Nm23多态性在湖北汉族肾移植患者中的分布

目的:研究Nm23基因中10个单核苷酸多态性(single nucleotide polymorphism,SNP)位点在湖北汉族肾移植患者中的分布情况。方法:采用实时荧光TaqMan-MGB探针等位基因分型技术对湖北汉族150例肾移植患者(男114例,女36例)和150例健康人群(男112例,女38例)的Nm23基因启动子及内含子区域共10个SNP位点的基因型进行检测。结果:Nm23基因上所选的10个SNP位点均符合Hardy-Weinberg平衡,其中rs7207370与rs1694969、rs34214448、rs2318785之间存在高度的连锁不平衡;rs8075231与rs2302254、rs2041296、rs8071647、rs2159359之间存在高度的连锁不平衡;并筛选出3个标签SNPs:rs7207370、rs8075231、rs11868380。结论:Nm23基因10个SNP位点的基因型及等位基因的分布,在肾移植患者和健康人群间无显著性差异。性别对基因型及等位基因的分布影响无统计学差异。

Distribution of polymorphism of anti-metastasis gene Nm23 in Hubei patients of Han nationality after renal transplantation in China

OBJECTIVE To study the distributions of 10 single nucleotide polymorphisms (SNPs) of Nm23 gene in Hubei Han patients after renal transplantation. METHODS The 10 SNPs of Nm23 gene in 150 renal transplant patients (114 men & 36 women) and 150 healthy subjects (112 men & 38 women) were genotyped by real time TaqMan-MGB genotyping assay. RESULTS The genotype distribution for the assayed loci followed the Hardy-Weinberg equilibrium. Strong linkage disequilibriums were observed in rs7207370 with rs1694969, rs34214448, and rs2318785, respectively; as well in rs8075231 with rs2302254, rs2041296, rs8071647 and rs2159359, respectively. And rs7207370, rs8075231, and rs11868380 were selected as Tag SNPs. CONCLUSION There are no significant differences in the genotypic and allelic distributions of SNPs between the renal transplant patients and healthy subjects. No significant differences in the distribution of Nm23 gene polymorphism were noted by gender.