鼻咽癌EGFL7的表达与肿瘤侵袭转移的关系

目的 观察表皮生长因子样结构域7(EGFL7)在鼻咽癌中的表达,并了解其与微血管密度(MVD)、淋巴管密度(LVD)的相关性,探讨其与鼻咽癌侵袭转移的关系及其可能的机制.方法 采用免疫组化法检测40例鼻咽癌组织(实验组)及20例鼻咽炎(对照组)中的EGFL7的表达;用CD31抗体及D2-40抗体分别检测实验组及对照组的MVD及LVD,并分析EGFL7的表达与肿瘤分级及MVD、LVD三者的相关性.结果 实验组与对照组均有EGFL7的表达,但实验组中EGFL7高表达率高于对照组(P<0.05);实验组中EGFL7的表达与MVD呈正相关,但与LVD相关性不明显;实验组中有淋巴结转移的LVD明显高于无淋巴结转移的(P<0.05);实验组中EGFL7的表达与患者性别、年龄、EB病毒抗体无相关性(P>0.05),与临床分期及有无淋巴结转移有关(P<0.05).结论 EGFL7并不像VEGF家族对血管、淋巴管均有双重作用,它仅表达于血管内皮细胞及鼻咽癌细胞中.EGFL7与鼻咽癌的生长和侵袭转移关系密切,它可能成为反映鼻咽癌转移及预后的标志物之一.

Relationship between the expression of EGFL7 in nasopharyngeal carcinoma and the invasion and metastasis of ;tumor

Objective To study the expression of epidermal growth factor-like domain 7(EGFL7) in nasopharyngeal carcinoma(NPC), and to investigate its correlation with microvessel density(MVD) and lymphatic vessel density(LVD) and its relationship with the invasion and metastasis of NPC and its possible mechanism. Methods The immunohisto chemical method was used to detect EGFL7 expression of 40 cases of nasopharyngeal carcinoma and 20 cases of nasopharyngitis; MVD and LVD were detected by antibody CD31 and D2-40 antibody for immunohistochemistry in the experimental and control groups, and to analyze the correlation of EGFL7 expression and tumor grade and MVD and LVD. Results The experimental group and the control group were of EGFL7 expression, but in the experimental group EGFL7 expression rate was higher than that of control group (P<0.05); EGFL7 expression and MVD was positively correlated in the experimental group, but LVD did not; in the experimental group, lymph node metastasis of LVD was significantly higher than those without lymph node metastasis of (P<0.05); in the experimental group, EGFL7 expression had no correlation with patient gender, age and EB virus antibody (P>0.05) but had some correlation with clinical stage and lymph node metastasis(P<0.05). Conclusion EGFL7 has no dual function on blood vessels and lymphatic vessels, it only expresses in endothelial cells and nasopharyngeal carcinoma cells. EGFL7 is closely associated with the growth and invasion and metastasis of nasopharyngeal carcinoma (NPC), it may become one of the markers reacting nasopharyngeal carcinoma metastasis and prognosis.