吡那地尔后处理对缺血/再灌注大鼠离体心脏超微结构的影响

目的观察吡那地尔后处理对缺血/再灌注成年大鼠离体心脏超微结构和线粒体评分的影响。方法 24只健康雄性SD大鼠,采用Langendorff离体心脏灌流系统,建立缺血/再灌注损伤模型,随机分为4组（n=6）,正常组（N）、缺血/再灌注组（I/R）、缺血后处理组（IPO）、吡那地尔后处理组（Pi）,使用透射电子显微镜,观察各组心肌组织超微结构变化,并进行线粒体评分。结果电镜显示心肌细胞N组结构正常,I/R组损伤最严重,IPO组及Pi组损伤程度相似,介于N组与I/R组之间。N组、IPO组、Pi组线粒体评分均低于I/R组（P〈0.05）。IPO组和Pi组线粒体评分无统计学意义（P=0.247）。结论缺血/再灌注可对心肌细胞超微结构造成严重损伤,使线粒体评分分值增加,造成心肌损害,缺血及吡那地尔后处理均可减轻再灌注所致心肌细胞超微结构损伤,降低线粒体评分分值,可能产生心肌保护作用。

Effects of pinacidil postconditioning on myocardial ultrastructure of adult rats cardiac ischemia / reperfusion in vitro

Objective To observe the effects of pinacidil postconditioning on myocardial ultrastructure and mitochondria score in isolated heart ischemia / reperfusion of adult rats. Methods The hearts of adult healthy male Sprague- Dawley rats were isolated and subject to ischemia reperfusion injury. Hearts were randomly divided into four groups（ n = 6 in each group） ： Normal group（ N）,ischemia- reperfusion group（ I / R）,ischemic postconditioning group（ IPO） and pinacidil postconditioning group（ Pi）. The ultrastructure and mitochondria score of myocardial tissue were observed by transmission electron microscope. Results Compared with those in I / R group,the damaged ultrastructure was attenuated and mitochondria score of myocardial cells were lower in IPO group and Pi group（ P〈0. 05）. No significant difference of mitochondria score of myocardial cells between IPO and Pi groups（ P = 0. 247） was detected. Conclusion Ischemia / reperfusion could induce the damage of myocardial cell ultrastructure. IPO group and Pi group have similarly protective effects on myocardium from ischemia /reperfusion in isolated rat hearts.