滤泡性淋巴瘤与反应性淋巴滤泡增生的免疫组化及DNA图像分析研究

目的：观察滤泡性淋巴瘤（ＦＬ）与反应性淋巴滤泡增生（ＲＦＨ）中滤泡生发中心细胞（ＧＣＣｓ）核ＤＮＡ含量、倍体情况以及ｂｃｌ－２癌基因蛋白、免球蛋白轻链（ＩｇＬ）表达情况，探讨它们对于二都鉴别诊断的意义。方法：对２１例ＦＬ及２１例ＲＦＨ进行ｂｃｌ－２蛋白、Ｋｆｐｐａ、Ｌａｍｂｄａ轻链蛋白免疫组化检测及ＤＮＡ图像细胞分析。结果：６１．９％ＦＬ中ＧＣＣｓ有ｂｃｌ－２表达而ＲＦＨ的ＧＣＣｓ均为ｂｃｌ－２阴

Immunohistochemical and DNA image cytometric study of follicular lymphoma and reactive follicular hyperplasia

Purpose:To investigate DNA content, DNA ploidy status and the expression of bcl 2 oncoprotein, immunoglobulin light chains(IgL) in follicular germinal center cells (GCCs) of follicular lymphoma (FL) and reactive follicular hyperplasia(RFH).Methods:Twenty one cases of FL and the same number cases of RFH were subjected to DNA image cytometry. And bcl 2 protein,Kappa,Lambda light chains were immunohistochemically detected. Results:The bcl 2 positivity of GCCs in FL and RFH were 61.9% and 0%,respectively ( P <0 005). And the restrictive expression of IgL was only seen in 6/12 Fl cases. Neoplastic GCCs have significantly higher mean DNA content than hyperplastic ones ( P <0 001). 66.7% FL cases were hyperdiploid while 90.5% RHF were diploid or hypodiploid. The variation of bcl 2 positivity and DNA content between different cytological grades of FL was noticed,too. Conclusions:Quantitative DNA analysis by image cytometry combined with bcl 2 immunohistochemical detection can be very helpful to the distinction between FL and RFH.