黄花乌头中Hetisine型生物碱的高速逆流色谱分离与结构鉴定

目的研究黄花乌头块根的化学成分，寻找更多天然活性物质。方法采用高速逆流色谱法分离纯化黄花乌头块根中的生物碱类化学成分，根据理化性质、波谱学分析鉴定化合物的结构。结果高速逆流色谱的两相溶剂分离系统采用氯仿-甲醇-0.2 mol·L^-1 HCl(体积比为10∶3∶3)，从黄花乌头块根中一次性分离得到8个化合物，分别鉴定为2α-propionyl-11α,13β-diacetyl-14-hydroxyhetisine (I)、关附巳素(II)、关附庚素(III)、关附己素(IV)、关附Z素(V)、关附辰素(VI)、关附甲素(VII)、关附壬素(VIII)。结论化合物I为新化合物，命名为关附未素(Guanfu base R)。

Isolation and identification of Hetisine-type alkaloids from Aconitum coreanum by high speed countercurrent chromatography

AIM: To search for more bioactive compounds from the roots of Aconitum coreanum (Lèvl.) Rapaics. METHODS: High speed countercurrent chromatography was successfully applied to the separation of alkaloids from Aconitum coreanum. The structures were elucidated by their physicochemical properties and spectroscopic analysis. RESULTS: Two-phase solvent system composed of CHCl3-CH3OH-0.2 mol x L(-1) HCl (10:3:3, volume ratio) was used in this experiment, eight alkaloids were obtained from the roots of Aconitum coreanum, which were identified as: 2alpha-propionyl-11alpha,13beta-diacetyl-14-hydroxyhetisine (I), Guanfu base P (II), Guanfu base G (III), Guanfu base F (IV), Guanfu base Z (V), Guanfu base O (VI), Guanfu base A (VII), Guanfu base B (VIII). CONCLUSION: Compound I is a new alkaloid, named Guanfu base R.