初次及再次感染HCV后不同功能区抗体的研究

１４例初次ＨＣＶ感染者及１１例再次ＨＣＶ感染者系列血清系在无法检测ＨＣＶ时留存的２８９位手术受血者系列血清中筛选获得。系列血清包括受血前、受血后不同时期收集的血清。回顾性地研究其不同功能区抗体出现的动态变化，抗Ｃ与抗ＮＳ３抗体有早期诊断价值。抗ＮＳ３、抗Ｃ、抗ＮＳ５及抗ＮＳ４抗体在ＨＣＶ感染后系列血清中检出率分别为８４．７６％、７９．２７％、７２．５４％和６８．３９％。感染过程中各区抗体可以全部出现、部分出现或单独出现抗Ｃ、抗ＮＳ３及抗ＮＳ５抗体，未发现单独含抗Ｅ、抗ＮＳ１及抗ＮＳ４区抗体的血清。抗Ｅ、抗ＮＳ１及抗ＮＳ４抗体消失较早。研究表明：以ＨＣＶＣ区、ＮＳ３区及ＮＳ５区编码的优势抗原包被的ＥＬＩＳＡ抗体诊断试剂盒将提高ＨＣＶ的诊断。

THE DYNAMIC STUDY FOR ANTIBODIES TO DIFFERENT REGION OF HCV GENOME

The serial sera were prospectively collected from 289 patients before HCV antibodies diagnostic kit was com-mercially available and detected retrospectively. 25 patients with HCV infection were found. 14 of them were pri-marily infected and 11 of them were repeatedly infected. The dynamics of antibodies to different reglons of HCVgenome was studied. Antibodies to C and NS3 regions of HCV genome could improve early diagnosis. Amtlbodiesto C,E,NS1,NS3.NS4 and NS5 of HCV genome could be found in 79. 27%,33.16%,22.80%,84.76%,68.39%and 7 54%respectively in serial sera. Antibodies to different regions of HCV genome could be found to-tally or partly. There were sera containing antibodies only to C ,NS3 and NS5 regions of HCV genome. The studyshowed that ELISA coated with HCv antigens of C,NS3 and NS5 would improve diagnosis of PTHC and screen-ing for blood donors.