| 多聚酶链反应检测恙虫病立克次体核酸 |
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| 引用本文： | 陈添胜 郭辉玉. 多聚酶链反应检测恙虫病立克次体核酸[J]. 中华微生物学和免疫学杂志, 1994, 14(2): 129-130 |
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| 作者姓名： | 陈添胜 郭辉玉 |
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| 作者单位： | 广东省流行病防治研究所，广州中山医科大学微生物学与免疫学教研室 |
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| 摘 要： | 作者设计合成一对恙虫病立克次体ｓｔａ５８基因的ＤＮＡ引物，分别从恙虫病立克次体ｋａｒｐ、Ｇｉｌｌｉａｍ及ＣＭＹ株基因组中扩增出１ｋｂ片段，普氏及莫氏立克次体、西伯利亚立克次体和贝氏柯克斯体则不能，表明该引物为恙虫病立克次体种特异。以含ｓｔａ５８基因片段的重组质粒为模板作敏感性鉴定，表明ＰＣＲ可检测到１０ｐｇ的靶ＤＮＡ。用该引物作ＰＣＲ检测实验感染恙虫病立克次体的小鼠血、腹水及脾标本均获满意结果。
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| 关 键 词： | 多聚酶链反应，恙虫病立克次体 |
DETECTION OF RICKETTSIA TSUTSUGAMUSHI DNA BY POLYMERASE CHAIN REACTION |
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| Chen Tian-sheng,Liu Zou-lu,Guo Hui-yu,et al. DETECTION OF RICKETTSIA TSUTSUGAMUSHI DNA BY POLYMERASE CHAIN REACTION[J]. Chinese Journal of Microbiology and Immunology, 1994, 14(2): 129-130 |
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| Authors: | Chen Tian-sheng Liu Zou-lu Guo Hui-yu et al |
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| Abstract: | DNA prinmers derived from the nucleotide sequence of sta58 gene of R.tsutsugamushi led to a 1-kb fragmentby amplifying the genomic DNAs from strains Karp ,Gilliam and CMY of R. tsutsugamushi ,but not from R.Prowazekii,R.mooseri,R.sibirica or Coxiella burnetii.Sensitivity test showed that as few as 10pg of the templateDNA could be detected by PCR.Specimens of blood ,ascites and spleen of mice have been tested.It indicates thatPCR technology is suitable for epidemiological research of scrub typhu |
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| Keywords: | Polyrnerase chain reaction Rickettsia tsutsugamushi |
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