幽门螺杆菌脂多糖生物学活性的研究

应用酚水法提取了幽门螺杆菌９０２９０５株的脂多糖（ＬＰＳ）。该ＬＰＳ在０．５ｎｇ／ｍｌ浓度时即能凝固鲎血液变形细胞溶解物，注入家兔后可使动物出现Ｓｈｗａｒｔｚｍａｎ现象和发热反应，其热曲线呈双峰型。该ＬＰＳ注入家兔后０．５小时动物血压明显下降，至注后１小时血压降为零，外周血中白细胞数则先升后降。小鼠体内注入该ＬＰＳ后，可导致动物死亡，其内脏出现严重的毛细血管扩张并伴有少量出血。与Ｅ．ｃｏｌｉＬＰＳ对比实验结果证实幽门螺杆菌ＬＰＳ具有典型的内毒素活性。

RESEARCH ON BIOLOGICAL ACTIVITY OF THE LIPOPOLYSACCHARIDE OF HELICOBACTER PYLORI

he lipopolysaccharide of H elicobacter pylori (Hp-LPS)was extracted with phenol-water method. Hp-LPS atthe concentration of 0.5ng/ml could solidify limulus amoebocyte lysate. Shwartzman phenomenon and pyrogeneticresponse with biphasic curve in rabbits would occur after the animals were given with Hp-LPS.The blood pressureof rabbits was obviously declined after the animals had been injected with Hp-LPS and the blood pressure fell to ze-ro at the first hour after injection.The leukocyte number in peripheral blood of the rabbits increased at first andthen decreased gradually. Hp-LPS could result in the death of the mice if Hp-LPS had been injected into theanimals.Serious capillarectasia with a slight hemorrhage in internal organs of the mice could been observed obvi-ously. The results showed that Hp-LPS possessed activity of endotoxin.