CD147和MMP-2在乳腺癌组织中的表达及其与预后的相关性

背景与目的已知基质金属蛋白酶(matrixmetalloproteinases,MMPs)在恶性肿瘤侵袭与转移中发挥重要作用,而CD147作为MMPs的诱导因子,可促进肿瘤及间质细胞表达MMPs。本研究旨在探讨CD147和MMP-2在乳腺癌及肿瘤周围组织中的表达与乳腺癌的侵袭、转移及预后的关系。方法用免疫组化EnVision法检测112例乳腺癌及其癌旁正常组织中MMP-2和CD147的表达。结果CD147在55例肿瘤组织中有表达,阳性率为49.1%(55/112),但在癌旁正常腺体及肿瘤间质细胞中无表达。而MMP-2在60例肿瘤周围正常间质细胞中有表达,阳性率为53.6%(60/112);在33.1%(37/112)的肿瘤组织中也有表达。MMP-2的表达与肿瘤大小、淋巴结转移和临床分期呈显著性正相关(P<0.01);CD147的表达与病理分级、淋巴结转移以及雌激素受体(estrogenreceptor,ER)的表达呈显著性正相关(P<0.01)。单因素生存分析显示CD147和MMP-2阳性组的总生存率低于阴性组(P<0.05)。多元回归分析证实CD147是风险因子,其表达越高患者生存期越短。结论MMP-2及其诱导因子CD147可能参与了乳腺癌的侵袭与转移;CD147可做为独立的预后判断因子,结合病理分级和临床分期分析能提高对乳腺癌患者预后判断的准确性。

Expressions of CD147 and matrix metalloproteinase-2 in breast cancer and their correlations to prognosis

BACKGROUND & OBJECTIVE: Matrix metalloproteinases (MMPs) play an important role in invasion and metastasis of cancers. CD147, an inducer of MMPs, can activate the expression of multiple MMPs in cancer cells and interstitial cells. This study was to investigate the correlations of CD147 and MMP-2 expressions in tumor and adjacent tissue to metastasis, invasion, and prognosis of breast cancer. METHODS: Expressions of CD147 and MMP-2 in 112 specimens of breast cancer and adjacent tissue were detected by EnVision immunohistochemistry. RESULTS: Positive rate of CD147 was 49.1% in breast cancer tissues, and 0 in adjacent tissues and interstitial cells. Positive rate of MMP-2 was 33.1% in breast cancer tissues, and 53.6% in normal adjacent tissues. The intensity of MMP-2 staining in normal tissue was positively related with tumor size, lymph node metastasis, and clinical stage (P < 0.01). CD147 staining in cancer tissue was positively related with lymph node metastasis, estrogen receptor(ER) expression, and pathologic grade (P < 0.01). Univariate analysis indicated that the overall survival rate was significantly lower in patients with positive expression of CD147 or MMP-2 than in patients with negative expression (P < 0.05). Multivariate analysis showed that CD147 was a risk predictor; the higher expression of CD147, the shorter survival time of the patients. CONCLUSIONS: MMP-2 and its inducer CD147 maybe participate in invasion and metastasis of breast cancer. CD147 may be an independent prognostic factor and, when used in combination with pathologic grading and clinical staging, may increase accuracy of predicting prognosis of patients with breast cancer.