| 程序性坏死在髁突软骨退变中的作用 |
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| 引用本文： | 何峰,叶涛,马原军,杨祖阁,李天乐,仝昕,于世宾.程序性坏死在髁突软骨退变中的作用[J].实用口腔医学杂志,2021(1):35-39. |
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| 作者姓名： | 何峰 叶涛 马原军 杨祖阁 李天乐 仝昕 于世宾 |
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| 作者单位： | 军事口腔医学国家重点实验室 |
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| 基金项目： | 国家自然科学基金面上项目(编号:81970953);陕西省重点研发计划一般项目(编号:2019SF-102);军事口腔医学国家重点实验室自主研究课题(编号:2018ZB02)。 |
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| 摘 要： | 目的:探究程序性坏死(Necroptosis)在髁突软骨退变中的作用.方法:48只SD大鼠随机分为对照(Con)组、雌激素补充(E2)组、单侧前牙反(UAC)组、单侧前牙反+雌激素补充(U+E2)组(n = 12),3周后处死、取材.HE、番红O-固绿染色评价软骨退变程度;免疫组化及Western blot检测髁突...
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| 关 键 词： | 髁突 软骨 程序性坏死 损伤相关分子模式 退行性变 |
The effects of necroptosis on the development of condylar cartilage degeneration |
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| HE Feng,YE Tao,MA Yuanjun,YANG Zuge,LI Tianle,TONG Xin,YU Shibin.The effects of necroptosis on the development of condylar cartilage degeneration[J].Journal of Practical Stomatology,2021(1):35-39. |
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| Authors: | HE Feng YE Tao MA Yuanjun YANG Zuge LI Tianle TONG Xin YU Shibin |
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| Institution: | (State Key Laboratory of Military Stomatology,National Clinical Research Center for Oral Diseases,Shaanxi International Joint Research Center for Oral Diseases,Department of Oral Anatomy and Physiology and TMD,School of Stomatology,the Fourth Military Medical University,Xi'an 710032,China) |
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| Abstract: | Objective:To explore the effects of necroptosis on the development of mandibular condylar cartilage degeneration in rats.Methods:48 SD rats were randomly divided into control(Con),estrogen supplement(E 2),unilateral anterior crossbite(UAC)and UAC with estrogen supplement(U+E 2)groups(n=12).All rats were sacrificed 3 weeks later.HE and Safranin O staining were performed to evaluate the degree of condylar cartilage degeneration.Immunohistochemical staining and western blot were used to detect the expression of crucial molecules of necroptosis(RIPK3,p-MLKL),damage associated molecular patterns(HMGB1)and pro-inflammatory and catabolic cytokines(TNF-α,MMP13,ADAMTS5)in cartilage.Results:Compared with Con group,there was a significant decrease in the thickness of cartilage and positive area of Safranin O staining(P<0.01),accompany with a significant increase in the percentage of positive chondrocytes and the expression of RIPK3,p-MLKL,TNF-αand HMGB1(P<0.05)in E 2,UAC and UAC+E 2 groups.The expression of MMP13 and ADAMTS5 was significantly higher in UAC and U+E 2 group than that in Con group(P<0.01).Conclusion:Necroptosis of condylar chondrocytes is involved in the development of condylar cartilage degeneration. |
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| Keywords: | Condylar Cartilage Necroptosis Damage associated molecular patterns Degenerative change |
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