特异性核基质结合区结合蛋白1和微小RNA-495-3P在甲状腺乳头状癌侵袭和转移中的作用

目的:探讨特异性核基质结合区结合蛋白1(SATB1)和微小RNA(microRNA，miR)-495-3P在甲状腺乳头状癌(PTC)侵袭和转移中的作用。方法:2019年9月至2020年4月，福建医科大学附属第二医院甲状腺乳腺外科采集肿瘤标本，将取得的40对PTC组织作为实验组，与之对应40对癌旁正常组织作为对照组。采用...

Roles of special adenine-thymine-rich-binding rich sequence binding protein-1 and microRNA-495-3P in invasion and metastasis of papillary thyroid carcinoma

Objective To study the role of special adenine-thymine-rich-binding rich special AT-rich-binding protein 1(SATB1)and microRNA(miRNA,miR)-495-3P in the invasion and metastasis of papillary thyroid carcinoma(PTC).Methods All specimens were collected from department of Thyroid and Breast Surgery,Second Affiliated Hospital of Fujian Medical University from September 2019 to April 2020,including 40 cases of PTC and 40 cases of corresponding normal thyroid tissues.There were 10 males and 30 females,aged 18-68 years,with a median age of 43 years.Quantitative real-time polymerase chain reaction(RT-qPCR)was used to detect the expression levels of SATB1 and miR-495-3p in 40 pairs of PTC tissues and normal tissues adjacent to cancer.Western blotting was used to detect the expression levels of SATB1 protein in 16 pairs of PTC tissues and normal tissues adjacent to cancer.After transfecting Human thyroid papillary carcinoma cells(TPC-1)with si-SATB1,RT-qPCR and Western blotting were used to detect the expression levels of SATB1 and miR-495-3p in TPC-1,and Pearson analysis method was used for correlation analysis.Methyl thiazol tetrazolium(MTT)method,flow cytometry and Trsnawell method were used to detect the effect of knocking down SATB1 expression on TPC-1 proliferation,apoptosis,cycle,invasion and migration.Results Compared with normal tissues adjacent to cancer,SATB1 mRNA and SATB1 proteins were highly expressed in PTC tissues(1.27±0.14 vs.0.86±0.23,t=8.484,P<0.01 and 0.94±0.10 vs.0.37±0.15,t=11.890,P<0.01),and miR-495-3p was down-regulated(0.78±0.11 vs.1.37±0.64,t=5.741,P<0.01).The abnormal expression of SATB1 and miR-495-3p was significantly correlated to PTC lymph node metastasis(1.34±0.14,t=2.576,P<0.05 and 0.74±0.07,t=2.187,P<0.05).The expression levels of SATB1 and miR-495-3p in PTC were negatively correlated(r=-0.497,P<0.01).Interfering with the expression of SATB1 in TPC-1 could increase the expression level of miR-495-3p(8.59±0.16 vs.1.01±0.02,t=81.420,P<0.01),and inhibit cell proliferation(0.39±0.01,0.52±0.01,0.58±0.03 vs.0.43±0.01,0.60±0.01,0.72±0.01,t=4.899,9.798,7.668,P<0.01),promote cell apoptosis(42.8±2.1)%vs.(7.6±0.7)%,t=27.540,P<0.01],weaken cell invasion(75.33±3.51 vs.206.33±5.51,t=34.730,P<0.01),migration(202.00±7.81 vs.528.33±5.03,t=60.840,P<0.01)and G2/M cycle block occurred(36.7±1.2)%vs.(15.6±0.9)%,t=24.360,P<0.01].Conclusion MiR-495-3p which may be used as a regulator factor and SATB1 jointly participate in the process of PTC invasion and metastasis.