黄芪皂苷对X线诱发骨髓间充质干细胞的基因损伤的保护作用

目的研究黄芪皂苷(AS)对X线辐照骨髓间充质干细胞(BMSCs)增殖水平影响及其诱发DNA损伤的保护作用。方法将细胞分为4组:空白组、AS组(50μg·mL^-1AS)、辐射组、辐射+AS组(50μg·mL^-1AS)。药物干预3 d后,辐射组、辐射+AS组用2 Gy X线进行照射。用CCK-8法检测各组细胞的增殖能力(OD值),微核法检测各组BMSCs的微核率,免疫荧光法检测53BP1焦点数。结果空白组、AS组、辐射组和辐射+AS组在5 d的增殖能力分别为1.26±0.06,1.16±0.03,0.88±0.02和0.96±0.03;这4组在0.5 h的53BP1焦点簇的数目分别为10.00±2.00,9.66±3.06,875.33±41.06和704.66±47.42;这4组的微核率分别为(0.40±0.17)%,(0.50±0.20)%,(5.93±0.97)%和(3.97±0.61)%。上述指标,辐射组与空白组相比,或辐射+AS组与辐射组相比,差异均有统计学意义(均P<0.05)。结论AS对X线辐射诱发人骨髓间充质干细胞的增殖及基因组DNA损伤有防护作用。

Protective effect of astragalus saponins on DNA damage induced by X ray in bone marrow mesenchymal stem cells

Objective To observe effect of astragalus saponins(AS)on proliferation of bone marrow mesenchymal stem cells(BMSCs)irradiated by X-ray and its protective effect on desoxyribonucleic acid(DNA)damage induced by X-ray irradiation.Methods The cells were divided into four groups:blank group,AS group(50μg·mL^-1AS),radiation group,radiation+AS group(50μg·mL^-1AS).The radiation group and radiation+AS group were irradiated with 2 Gy X-rays after intervention for 3 d.Cell proliferation ability(OD value)was detected by CCK-8 method.The micronucleus rate of BMSCs in each group was detected by micronucleus method.The 53 BP1 foci number were detected by immunofluorescence method.Results The proliferation ability of blank group,AS group,radiation group and radiation+AS group at fifth day were 1.26±0.06,1.16±0.03,0.88±0.02 and 0.96±0.03,respectively;the number of 53 BP1 foci in the four groups at 0.5 h were 10.00±2.00,9.66±3.06,875.33±41.06 and 704.66±47.42;the micronucleus rates of the four groups were(0.40±0.17)%,(0.50±0.20)%,(5.93±0.97)%and(3.97±0.61)%respectively.Compared between radiation group and blank group,or radiation+AS group and radiation group,the differences of the factors were statistically significant(all P<0.05).Conclusion AS has protective effect on X-ray DNA induced damage of BMSGs.