| 钩藤碱对大鼠肺动脉平滑肌细胞钙激活钾通道的影响 |
| |
| 引用本文： | 开丽,王中峰. 钩藤碱对大鼠肺动脉平滑肌细胞钙激活钾通道的影响[J]. 中国药理学与毒理学杂志, 1999, 13(1): 33-36 |
| |
| 作者姓名： | 开丽 王中峰 |
| |
| 作者单位： | 第三军医大学基础医学部药理学教研室 |
| |
| 摘 要： | 用膜片钳单通道记录法研究钩藤碱(Rhy)对大鼠肺动脉平滑肌细胞钙激活钾通道(KCa)的影响.结果：Rhy 30， 45和60 μmol·L-1缩短通道的开放时间， 但浓度依赖性增加KCa开放概率，Rhy 15， 30， 45和60 μmol·L-1使开放概率由加药前的0.085±0.005分别增加到0.176±0.011， 0.315±0.009， 0.485±0.016和0.761±0.012(x±s, 均P<0.01).说明Rhy有增加肺动脉平滑肌细胞KCa开放作用.
|
| 关 键 词： | 钩藤碱；钾通道；膜片钳技术；肌 平滑 血管；肺动脉 |
| 收稿时间： | 1997-09-02 |
Effect of rhynchophylline on calcium-activated potassium channels in isolated rat pulmonary smooth muscle cells |
| |
| KAI Li, WANG Zhong-Feng. Effect of rhynchophylline on calcium-activated potassium channels in isolated rat pulmonary smooth muscle cells[J]. Chinese Journal of Pharmacology and Toxicology, 1999, 13(1): 33-36 |
| |
| Authors: | KAI Li WANG Zhong-Feng |
| |
| Affiliation: | (1. Department of Pharmacology, 2. Department of Physiology, Faculty of Basic Medical Sciences, Third Military Medical University, Chongqing 400038) |
| |
| Abstract: | Using inside-out configuration of patch-clamp single channel recording technique, the effect of rhynchophylline(Rhy) on calcium activated potassium channels(KCa) in isolated rat pulmonary smooth muscle cells was studied. The results showed that Rhy 30, 45 and 60 μmol·L-1 decreased the open time, but increased the open probability of KCa in concentration-dependent manner. Rhy 15, 30, 45 and 60 μmol·L-1 increased the open probability of KCa from control value 0.085±0.005 to 0.176±0.011, 0.315±0.009, 0.485±0.016 and 0.761±0.012, respectively (all P<0.01). The results indicate that Rhy has opening effects on KCa in isolated rat pulmonary smooth muscle cells. |
| |
| Keywords: | rhynchophylline potassium channels patch clamp technique muscle smooth vascular pulmonary artery |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国药理学与毒理学杂志》浏览原始摘要信息 |
|
点击此处可从《中国药理学与毒理学杂志》下载全文 |
