| 氯化镍对人红细胞膜损伤机理的波谱学研究 |
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| 引用本文： | 周晓,刘世杰,陈建文,江泉观,高松,陆敬泽,周永芬. 氯化镍对人红细胞膜损伤机理的波谱学研究[J]. 中国药理学与毒理学杂志, 1999, 13(1): 65-68 |
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| 作者姓名： | 周晓 刘世杰 陈建文 江泉观 高松 陆敬泽 周永芬 |
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| 作者单位： | 北京医科大学劳动卫生学教研室，北京放射医学研究所，中国科学院生物物理研究所生物膜研究室，北京大学稀土化学研究中心 |
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| 摘 要： | 为探讨镍对红细胞膜损伤作用的机理，采用傅立叶变换红外吸收技术和园二色光谱技术，分别研究了氯化镍对人红细胞膜蛋白和重组于卵磷脂脂质体上ｂａｎｄ３蛋白的二级结构影响，同时采用５－脱氧不饱和硬脂酸标记的电子自旋共振技术，研究了膜脂质流动性的变化．结果表明：在１－１００μｍｏｌ·Ｌ－１浓度范围内，镍离子可使红细胞膜蛋白发生变性和不可逆聚合，使膜浅层脂质流动性增加；０．０５，０．１０，０．５０ｍｍｏｌ·Ｌ－１的镍可使ｂａｎｄ３的α－螺旋和无规卷曲含量分别有减少和增加的趋势．
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| 关 键 词： | 氯化镍；红细胞膜；蛋白质构象；膜流动性 |
| 收稿时间： | 1997-04-14 |
Spectrum studies on the mechanisms of human erythrocyte membrane injuries induced by nickel chloride |
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| ZHOU Xiao,LIU Shi Jie,CHEN Jian Wen,JIANG Quan Guan,GAO Song,LU Jing Ze,ZHOU Yong Fen. Spectrum studies on the mechanisms of human erythrocyte membrane injuries induced by nickel chloride[J]. Chinese Journal of Pharmacology and Toxicology, 1999, 13(1): 65-68 |
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| Authors: | ZHOU Xiao LIU Shi Jie CHEN Jian Wen JIANG Quan Guan GAO Song LU Jing Ze ZHOU Yong Fen |
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| Affiliation: | (Department of Occupational Health, Beijing Medical University, Beijing 100083) |
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| Abstract: | Changes of conformation of human erythrocyte membrane proteins were investigated with Fourier-transform infrared absorbance spectroscopy and circular dichroism. The fluidity of membrane lipid was studied with 5-deoxyl-stearic acid labeling electron spin resonance spectroscopy. The results showed that nickel chloride aqueous solution caused the denaturation and coagulation of membrane proteins as well as the increase of membrane lipid fludity at concentration of 1.0-100 μmol·L-１; at concentration of 0.05, 0.1, 0.5 mmol·L-１, nickel might induce the change of the secondary structure, there were tendencies to reduction of α-helix content and increase of the content of unordered structure. |
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| Keywords: | nickel chloride erythrocyte membrane protein conformation membrane fluidity |
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