兔膝关节软骨缺损修复的初步研究

目的　通过髓腔细胞的分化、生长及应用生长因子 ,修复穿透软骨下骨的关节软骨损伤。方法　采用胶原凝胶与髓腔细胞相混 ,植入兔膝关节实验性软骨缺损区 ,并加入少量细胞生长因子 ,观察软骨缺损修复情况。结果　胶原凝胶移植后的修复组织为透明软骨样组织 ,表面光滑 ,基底部骨小梁形成良好 ,且与碱性成纤维细胞生长因子相混合后 ,其修复组织生长似乎更好一些 ;而单纯钻孔组的修复则是不完全的 ,形成以纤维组织为主的修复组织。结论　关节软骨下髓腔内的间叶细胞在胶原凝胶载体支架的保护下 ,关节腔内特定的局部微环境使其向软骨细胞转化 ,形成修复组织

Preliminary Experimental Study on Repair of Defects in Articular Cartilage of Rabbit Knee

Objective Injuries of the articular cartilage penetrated the subchondral bone were repaired by the bone-marrow cells and the growth factor.Methods The mixture of collagen gel and marrow cells,partly with some growth factor,was implanted into the experimental defects of articular cartilage in rabbit knees.Results The reparative tissure in the defects belongs to hyaline cartilage with smooth surface and with good cancellous bone at the base. The reparative tissue appeared better with the use of the growth factor (bFGF).The reparation of defect was not complete in the group after simple drilling.The nature of reparative tissue was fibrous tissue.Conclusion The bone-marrow-derived mesenchymal cells with the protection by the collagen gel carrier can differentiate into the chondrocytes in the special micro-circumstances of joint and the defect may be repaired.