不同产地加工方法对野菊花中二咖啡酰奎尼酸类成分含量的影响

目的：建立测定野菊花中二咖啡酰奎尼酸类成分含量的方法，并比较不同产地加工方法对其含量的影响，为确定其较佳加工方法提供依据。方法：采用高效液相色谱法。色谱柱为DiamonsilC18（250mm×4．6mm，5μm），流动相为0．1％磷酸溶液。甲醇（梯度洗脱），检测波长为326nm，柱温为25℃，流速为0．8ml／min。结果：3，4-二咖啡酰奎尼酸、3，5．二咖啡酰奎尼酸和4，5-二咖啡酰奎尼酸的进样量分别在87．36~873．60、65．81~658．10、32．00~320．00ng范围内与各自峰面积积分值呈良好的线性关系（，分别为0．9999、0．9999、0．9998）；精密度、稳定性、重复性试验的RSD〈2％；平均加样回收率分别为99．59％、99．65％、99．50％，RSD分别为1．53％、1．78％、2．27％（n均为6）。结论：该方法操作简便、结果准确、重复性好，可用于野菊花中二咖啡酰奎尼酸类成分的含量测定。野菊花中二咖啡酰奎尼酸类成分的含量除与温度有关外，还与烘晒时间长短有一定的关系。

Effects of Different Producing Area Processing on the Content of Dicaffeoylquinic Acid in Chrysanthemum indicum

OBJECTIVE： To establish the method for the content determination of dicaffeoylquinic acid in Chrysanthemum indi- cum and compare the effects of different producing area processing on the content of dicaffeoylquinic acid, and to provide reference for determining the optimal processing technology. METHODS： HPLC method was used. The separation was performed on Di- amonsil C18（250 mm×4.6 mm, 5 μm）colunm with mobile phase consisted of 0.1% phosphoric acid-methanol （gradient elution） at flow rate of 0.8 ml/min. The detection wavelength was set at 326 nm and column temperature was 25 ℃. RESULTS： The linear ranges were 87.36-873.60 ng （r= 0.999 9） for 3,4-O-dicaffeoylquinic acid, 65.81-658.10 ng （r = 0.999 9 ） for 3,5-O-dicaffeoylquinic acid and 32.00-320.00 ng （r=0.999 8）for 4, 5-O-dicaffeoylquinic acid. The RSDs of precision, stablility and reproducibility tests were all lower than 3%. Average recoveries were 99.59% （RSD=1.53%, n=6）, 99.65% （RSD=1.78%, n=6） and 99.50% （RSD = 2.27 %, n = 6）, respectively. CONCLUSIONS： The method is simple, accurate and reproducible, and it is suitable for the content determination of dicaffeoylquinic acid in C. indicum. The content of dicaffeoylquinic acid in C. indicum is related with tem- perature and also associated with drying time.