| 饿蚂蝗中多酚的含量测定 |
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| 引用本文： | 袁见萍,张龙,张前军,康文艺,周清娣. 饿蚂蝗中多酚的含量测定[J]. 中国药房, 2013, 0(43): 4078-4080 |
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| 作者姓名： | 袁见萍 张龙 张前军 康文艺 周清娣 |
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| 作者单位： | [1]贵州大学精细化工研究开发中心,贵阳550025 [2]贵州大学化学与化工学院,贵阳550025 [3]河南大学中药研究所,河南开封475004 [4]澳大利亚悉尼大学化学学院,悉尼1797 |
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| 基金项目： | 基金项目：教育部“春晖计划”立项资助合作科研项目（No.Z2011036）;贵阳市科技计划项目（No.筑科合同[20122041号） |
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| 摘 要： | 目的：建立测定饿蚂蝗中多酚含量的方法。方法：以没食子酸为对照品，采用优化后的Folin．Ciocateau比色法（考察了测定波长、碳酸钠溶液体积、Folin．Ciocateau显色剂加入量、反应温度及时间等）测定饿蚂蝗不同提取物中多酚的含量。结果：没食予酸的质量浓度在0．001O～0．0061mg／ml范围内与其吸光度呈良好的线性关系（r=0．9994）；平均加样回收率为99．99％，RSD=0．59％（n=6）。饿蚂蝗75％乙醇提取物中多酚的质量分数最高，为9．83％；其次为40％乙醇提取物，多酚质量分数为8．55％；80％乙醇提取物中多酚的质量分数最低，为0．32％。结论：采用优化后的Folin—Ciocateau比色法测定饿蚂蝗中多酚的含量，精密度和灵敏度高、稳定性和重复性好、操作简单。
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| 关 键 词： | 饿蚂蝗 多酚 Folin-Ciocateau比色法 含量测定 |
Content Determination of Polyphenols in Desmodium sambuense |
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| YUAN Jian-ping,ZHANG Long,ZHANG Qian-jun,KANG Wen-yi,ZHOU Qing-di. Content Determination of Polyphenols in Desmodium sambuense[J]. China Pharmacy, 2013, 0(43): 4078-4080 |
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| Authors: | YUAN Jian-ping ZHANG Long ZHANG Qian-jun KANG Wen-yi ZHOU Qing-di |
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| Affiliation: | 1.Fine Chemical R＆D Center, Guizhou University, Guiyang 550025, China; 2. College of Chemistry and Engineering, Guizhou Uni- versity, Guiyang 550025, China; 3. Institute for TCM Research, Henan University, Henan Kaifeng 475004, China; 4. College of Chemistry, Sydney University,Sydney 1797, Australia) |
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| Abstract: | OBJECTIVE： To establish the method for the content determination ofpolyphenols in Desmodium sambuse. METH- ODS ： Using gallic acid as control, the content of polyphenols in different extracts of D. sambuense was determined by Folin-Cioca- teau colorimetry. The absorption wavelength, the volume of sodium carbonate, the amount of Folin-Ciocalteu regent, reaction tem- perature and time were explored. RESULTS： The linear range of gallic acid were 0.001 0-0.006 1 mg/ml（r=0.999 4） with an aver- age recovery of 99.99% （RSD=0.59%, n：6）. The mass fraction of polyphenols in 75% ethanol extracts of D. sambuense was the highest, being 9.83 %; followed by 40 % ethanol extracts, being 8.55 %; that of polyphenols in 80% ethanol extract was the lowest （0.32%）.CONCLUSIONS： Optimized Folin-Ciocateau colorimetry is adopted to determine the content of polyphenols in D. sam- buense, whichi is simple, stable and repeatbale with high precision and sensitivity. |
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| Keywords: | Desmodium sambuense Polyphenols Folin-Ciocateau colorimetry Content determination |
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