HPLC法同时测定红花注射液中腺苷和羟基红花黄色素A的含量

目的：建立同时测定红花注射液中腺苷和羟基红花黄色素A含量的方法。方法：采用高效液相色谱法。色谱柱为AgilentEclipSeXDB—C18（250mm×4．6mm，5μm），流动相为甲醇一乙腈．0．7％磷酸溶液（梯度洗脱），柱温为30℃，流速为0．8ml／min，检测波长为260nm（0~14min）和403nm（〉14~35min）。结果：腺苷和羟基红花黄色素A的质量浓度分别在1．194～71．640、2．070~82．800gg／ml范围内与各自峰面积积分值呈良好的线性关系（r均为0．9999）；精密度、重复性、稳定性试验的RSD〈1％；平均加样回收率分别为101．18％和100．56％，RSD分别为1．33％和1．13％（H均为9）。结论：该方法简便、准确、重复性好，可用于红花注射液的质量控制。

Content Determination of Adenosine and Hydroxysafflor Yellow A in Honghua Injections by HPLC

OBJECTIVE： To develop a method for the content determination of adenosine and hydroxysafflor yellow A in Hong- hua injection. METHODS： HPLC method was adopted. The determination was performed on Agilent Eclipse XDB-C18（250 mm× 4.6 mm, 5 μm）with mobile phase composed of methanol-acetonitrile-0.7%phosphoric acid （gradient elution） at the flow rate of 0.8 ml/min. The column temperature was 30 ℃. The detection wavelength was set at 260 nm within 0-14 min and 403 nm within 14-35 min. RESULTS： The linear ranges of adenosine and hydroxysafflor yellow A were 1.194-71.640 μg/ml（r=0.999 9） and 2.070-82.800 μg/ml（r=0.999 9）. RSDs of precision, reproducibility and stability tests were all lower than 1%. The average recov- eries were 101.18% （RSD=1.33% ,n=9） and 100.56% （RSD=1.13% ,n=9）. CONCLUSIONS： The methods is simple, accurate and repeatable, and it is suitable for the guality control of Honghua injections.