改良乌头汤减毒增效比较研究

目的：研究不同配伍下乌头汤毒性生物碱成分的变化及其镇痛作用、急毒性。方法：采用高效液相色谱法测定不同配伍下乌头汤中苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰新乌头原碱的含量变化；采用醋酸致小鼠扭体模型，比较研究不同配伍下乌头汤的镇痛作用；研究不同配伍下乌头汤的急毒性。结果：与制川乌单煎比较，改良乌头汤（乌头原汤加防风、黑豆）中苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰新乌头原碱含量分别降低了73．09％、68．01％、74．53％；17．50、8．75、4．35g／kg改良乌头汤与12．50、6．25、3．13g／kg乌头原汤可明显减少醋酸致小鼠20min内扭体次数（P〈0．01或P〈0．05），17．50g／kg改良乌头汤与12．50、6．25g／kg乌头原汤可显著延长扭体潜伏期（P〈0．05）；改良乌头汤无急毒性反应。结论：乌头原汤配伍防风、黑豆能减少乌头原汤毒性生物碱成分的含量，鸟头原汤与改良乌头汤在镇痛药效方面无明显差异。

Comparative Study on Toxicity Attenuation and Efficacy Potentiation of Modified Wutou Decoction

OBJECTIVE： To study the change of toxic alkaloid in different compatibility of Wutou decoction and its pharmaco- dynamics and acute toxicity. METHODS： HPLC was applied to determine the contents of benzoylmesaconine, benzoylaconine, ben- zoylhypacoitine of Wutou decoction. Analgesic actions of different compatibility of Wutou decoction were compared by the methods of glacial acetic acid induced writhing test; the acute toxicity of it was studied. RESULTS： Compared with Processed Radix Aconiti Praeparat extract, the contents of benzoylaconine, benzoylhypacoitine, benzoylmesaconine decreased by 73.91% , 68.01, 7.53 in modified Wutou decoction（Wutou decoction combined with Saposhnikovia. divaricata and Glycine max）respectively; modified Wut- ou decoction （17.50,8.75,4.35 g/kg）and Wutou decoction （12.50,6.25,3.13 g/kg）could reduce writhing response within 20 min sig- nificantly（P〈0.01 or P〈0.05）. modified Wutou decoction （17.50 g/kg）and Wutou decoction （12.50,6.25 g/kg ）could extend the writhing latency of mice, but there was no significant difference in analgesic action. No acute toxic reaction was found.CONCLU- SIONS： Wutou decoction combined with S. divaricata and G. max could reduce the content of toxic alkaloid, but the analgesic ef- fect has no changes.