三七中与牛血清白蛋白结合的活性成分研究

目的：研究三七中与牛血清白蛋白（BSA）有结合作用的潜在活性成分。方法：采用平衡透析与高效液相色谱联用技术筛选三七中与BSA有结合作用的成分；考察BSA浓度、缓冲液pH值对三七皂苷与BSA结合作用的影响；比较三七中主要皂苷单体与BSA单独作用和三七总皂苷与BSA作用的区别。结果：三七中有3个化学成分与BSA结合作用明显，分别为人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1；各皂苷单独结合作用强于联合结合作用，各皂苷在与BSA结合过程中存在竞争作用。结论：蛋白结合．平衡透析．高效液相色谱法联用可有效、快速地预测三七中多种成分在体内的吸收情况，筛选其潜在活性成分，且能体现中药整体作用的特点。

Study on Active Ingredients in Panax notoginseng Combined with Bovine Serum Albumin

OBJECTIVE： To study potential active ingredients in Panax notoginseng combined with bovine serum albumin （BSA）. METHODS： The synergistic action components in P. notoginseng combined with BSA was studied by equilibrium dialysis coupled with HPLC. The effects of the concentration of BSA and pH value on their bindings were also investigated; the binding be- tween sanchinoside monomers of P notoginseng and BSA was compared with that between total saponins of P notoginseng and BSA. RESULTS： Result showed that 3 components of P notoginseng had combined with BSA, including ginsenosides RgL, ginsen- oside Re and ginsenoside Rbl. The binding degrees of sanchinoside monomers were better than those of total saponins; the sanchino- side monomers acted competitively each other in binding BSA process. CONCLUSIONS： Protein binding-equilibrium dialy- sis-HPLC is rapid and effective to predict the absorption of various components of P notoginseng, screen potential active ingredi- ents and present the characteristics of TCM.