肿节风药材的HPLC指纹图谱研究及其异嗪皮啶与迷迭香酸的含量测定

目的：研究肿节风药材的高效液相色谱（HPLc）指纹图谱，并测定药材中异嗪皮啶与迷迭香酸的含量，以建立药材有效的质量评价方法。方法：色谱柱为Inertsil ODS-4（250mm×4．6mm，5μm），流动相为0．1％磷酸溶液．乙腈（梯度洗脱），流速为1．0ml／min，检测波长为342nm。结果：所得肿节风药材的HPLC指纹图谱分离度、精密度、重复性和稳定性均良好；10批肿节风药材指纹图谱的平均相似度为0．853，不同产地样品的指纹图谱差异不大，但4个代表性色谱峰的峰面积有所区别。异嗪皮啶与迷迭香酸的质量浓度均在5-500mg／ml范围内与各自峰面积积分值呈良好的线性关系（r分别为0．9995、0．9989）；平均加样回收率分别为105．20％、99．52％，RSD分别为1．39％、1．56％（n均为6）；10批肿节风药材中异嗪皮啶与迷迭香酸的含量存在明显差异，尤以迷迭香酸含量差异较大。结论：该方法简便、准确、重复性好，可以作为肿节风药材的质量控制方法。

Analysis of HPLC Fingerprints and Content Determination of Isofraxidin and Rosmarinic Acid in Sarcandra glabra

OBJECTIVE： To study HPLC fingerprints of Sarcandra glabra, and to determine the contents of isofraxidin and rosmarinic acid and establish the method for quality evaluation. METHODS： The samples were separated by Inertsil ODS-4 （250 mm× 4.6 mm, 5 μm） column with mobile phase consisted of 0.1% phosphoric acid-acetonitrile （gradient elution） at the flow rate of 1.0 ml/ min. The detection wavelength was set at 342 nm. RESULTS： HPLC fingerprint of S. glabra was established with good separation, precision, repeatability and stability; average similarity of 10 batches of S. glabra was 0.853; there was no significant difference in fingerprint of samples from different producing areas, but the contents of 4 representative peaks were different from one another. The linear ranges of isofraxidin were 5-500 mg/ml （r=0.999 5） and rosmarinic acid were 5-500 mg/ml （r=0.998 9）. Average recoveries were 105.20% （RSD=1.39%, n=6） and 99.52% （RSD=1.56%, n=6）. The contents of isofraxidin and rosmarinic acid from 10 batches were obviously different from one another, especially rosmarinic acid. CONCLUSIONS： The method is simple, accurate and repeatable, which can be used for the quality control of S. glabra.